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United States (US)

GABA B Receptor 1 (phospho Ser923) antibody

GABAB-Receptor-phospho-Ser923-antibody-R1-Subunit-GTX31018-WB-1_18121410_550.jpg

Cat No. GTX31018

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Rat
APPLICATION

Application Note

Optimal working dilutions should be determined experimentally by the end user.

Calculated MW

102 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

100 μl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Rat

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser923 conjugated to KLH

Purification

Affinity Purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

dJ271M21.1.1, gammaaminobutyric acid (GABA) B receptor, 1, GABBR13, GABA B Receptor1, dJ271M21.1.2, GPRC3A, GABBR1, GB1, GABBR1-3, GABABR1, FLJ92613, gamma-aminobutyric acid (GABA) B receptor, 1, GABAB Receptor (phospho S923), GABAB Receptor (phospho Ser923), GABAB Receptor (pS923), GABAB Receptor (pSer923), GABAB Receptor phospho S923, GABAB Receptor phospho Ser923, Phospho GABAB Receptor (S923), Phospho GABAB Receptor (Ser923), Phospho GABAB Receptor (pS923), Phospho GABAB Receptor (pSer923)

Cellular Localization

Moreover coexpression of GABA-B-R1 and GABA-B-R2 appears to be a prerequisite for maturation and transport of GABA-B-R1 to the plasma membrane. Colocalizes with ATF4 in hippocampal neuron dendritic membranes.

Background

Receptor for GABA. The activity of this receptor is mediated by G-proteins that inhibit adenylyl cyclase activity, stimulates phospholipase A2, activates potassium channels, inactivates voltage-dependent calcium-channels and modulates inositol phospholipids hydrolysis. Plays a critical role in the fine-tuning of inhibitory synaptic transmission. Pre-synaptic GABA-B-R inhibit neurotransmitter release by down-regulating high-voltage activated calcium channels, whereas postsynaptic GABA-B-R decrease neuronal excitability by activating a prominent inwardly rectifying potassium (Kir) conductance that underlies the late inhibitory postsynaptic potentials. Not only implicated in synaptic inhibition but also in hippocampal long-term potentiation, slow wave sleep, muscle relaxation and antinociception.

Database

Research Area

DATA IMAGES
GABAB-Receptor-phospho-Ser923-antibody-R1-Subunit-GTX31018-WB-1_18121410_550.jpg

GTX31018 WB Image

Western Blot: GABA B Receptor 1 [phospho Ser923] Antibody [GTX31018] - Rat synaptic membrane showing specific immunolabeling of the ~102k GABAB R1 protein phosphorylated at Ser923 (control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lambda-Ptase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the phospho-Ser923 GABAB antibody. The immunolabeling is completely eliminated by treatment with lambda-Ptase.

Package List Price ($)
$ 329