GeneTex
United States (US)

GABA B Receptor 2 antibody [N2C1], Internal

Overexpression
GTX105986_40506_20160921_IHC-Fr_19011506_740.jpg
GTX105986_40506_WB_19011506_140.jpg
GTX105986_40506_20180126_WB_B_19011506_560.jpg

Cat No. GTX105986

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-Fr
Reactivity Human, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-Fr 1:100-1:1000

Note :

WB
As is commonly seen with membrane proteins, significant hydrophobicity can lead to aggregation following boiling of samples prior to SDS-PAGE and subsequent western blotting. We recommend to avoid boiling in this case.

Not tested in other applications.

Calculated MW

106 kDa. ( Note )

Positive Control

U87-MG , unboiled Myc-DDK-tagged Gabbr2-transfected 293T

Predict Reactivity

Mouse(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.83mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human GABA B Receptor 2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Gamma-Aminobutyric Acid Type B Receptor Subunit 2,Eiee59,Gababr2,Gpr51,Gprc3B,Hg20,Hrihfb2099,Ndplhs,Gabbr2

Cellular Localization

Cell membrane; Multi-pass membrane protein , Cell junction , synapse , postsynaptic cell membrane; Multi-pass membrane protein

Background

B-type receptors for the neurotransmitter GABA (gamma-aminobutyric acid) inhibit neuronal activity through G protein-coupled second-messenger systems, which regulate the release of neurotransmitters and the activity of ion channels and adenylyl cyclase. See GABBR1 (MIM 603540) for additional background information on GABA-B receptors.[supplied by OMIM]

Database

Research Area

DATA IMAGES
GTX105986_40506_20160921_IHC-Fr_19011506_740.jpg

GTX105986 IHC-Fr Image

GABA B Receptor 2 antibody [N2C1], Internal detects GABA B Receptor 2 protein expression by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: GABA B Receptor 2 protein stained by GABA B Receptor 2 antibody [N2C1], Internal (GTX105986) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX105986_40506_WB_19011506_140.jpg

GTX105986 WB Image

Sample (30 ug of whole cell lysate)
A: U87-MG
7.5% SDS PAGE
GTX105986 diluted at 1:1000

GTX105986_40506_20180126_WB_B_19011506_560.jpg

GTX105986 WB Image

Overexpression

Non-transfected (–) and transfected (+) boiled and unboiled 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GABA B Receptor 2 antibody [N2C1], Internal (GTX105986) diluted at 1:2000 and DDDDK tag antibody (GTX115043) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169