APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
|
Note :
WB
As is commonly seen with membrane proteins, significant hydrophobicity can lead to aggregation following boiling of samples prior to SDS-PAGE and subsequent western blotting. We recommend to avoid boiling in this case.
|
Not tested in other applications.
Calculated MW
Positive Control
U87-MG , unboiled Myc-DDK-tagged Gabbr2-transfected 293T
Predict Reactivity
Mouse(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 20% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.83 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human GABA B Receptor 2. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_11172858
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
gamma-aminobutyric acid type B receptor subunit 2 , EIEE59 , GABABR2 , GPR51 , GPRC3B , HG20 , HRIHFB2099 , NDPLHS
Cellular Localization
Cell membrane; Multi-pass membrane protein , Cell junction , synapse , postsynaptic cell membrane; Multi-pass membrane protein
Background
B-type receptors for the neurotransmitter GABA (gamma-aminobutyric acid) inhibit neuronal activity through G protein-coupled second-messenger systems, which regulate the release of neurotransmitters and the activity of ion channels and adenylyl cyclase. See GABBR1 (MIM 603540) for additional background information on GABA-B receptors.[supplied by OMIM]
Database
Research Area
DATA IMAGES
![Anti-GABA B Receptor 2 antibody [N2C1], Internal used in IHC (Frozen sections) (IHC-Fr). GTX105986](/templates/default/js/lightbox/images/loading.gif "Anti-GABA B Receptor 2 antibody [N2C1], Internal used in IHC (Frozen sections) (IHC-Fr). GTX105986")
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GTX105986 IHC-Fr Image
GABA B Receptor 2 antibody [N2C1], Internal detects GABA B Receptor 2 protein expression by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: GABA B Receptor 2 protein stained by GABA B Receptor 2 antibody [N2C1], Internal (GTX105986) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
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GTX105986 WB Image
Non-transfected (–) and transfected (+) boiled and unboiled 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GABA B Receptor 2 antibody [N2C1], Internal (GTX105986) diluted at 1:2000 and DDDDK tag antibody (GTX115043) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX105986 WB Image
Sample (30 ug of whole cell lysate)
A: U87-MG
7.5% SDS PAGE
GTX105986 diluted at 1:1000
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REFERENCE
There are currently no references for GABA B Receptor 2 antibody [N2C1], Internal (GTX105986). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for GABA B Receptor 2 antibody [N2C1], Internal (GTX105986). Be the first to share your experience with this product.
![Anti-GABA B Receptor 2 antibody [N2C1], Internal used in IHC (Frozen sections) (IHC-Fr). GTX105986](/upload/website/prouct_img/small/GTX105986/GTX105986_40506_20160921_IHC-Fr_w_23060120_534.webp "Anti-GABA B Receptor 2 antibody [N2C1], Internal used in IHC (Frozen sections) (IHC-Fr). GTX105986")
![Anti-GABA B Receptor 2 antibody [N2C1], Internal used in Western Blot (WB). GTX105986](/upload/website/prouct_img/small/GTX105986/GTX105986_40506_20180126_WB_B_w_23060120_124.webp "Anti-GABA B Receptor 2 antibody [N2C1], Internal used in Western Blot (WB). GTX105986")
![Anti-GABA B Receptor 2 antibody [N2C1], Internal used in Western Blot (WB). GTX105986](/upload/website/prouct_img/small/GTX105986/GTX105986_40506_WB_w_23060120_928.webp "Anti-GABA B Receptor 2 antibody [N2C1], Internal used in Western Blot (WB). GTX105986")
![Anti-GABA B Receptor 2 antibody [N2C1], Internal used in IHC (Frozen sections) (IHC-Fr). GTX105986](/upload/website/prouct_img/normal/GTX105986/GTX105986_40506_20160921_IHC-Fr_w_23060120_534.webp "Anti-GABA B Receptor 2 antibody [N2C1], Internal used in IHC (Frozen sections) (IHC-Fr). GTX105986")
![Anti-GABA B Receptor 2 antibody [N2C1], Internal used in Western Blot (WB). GTX105986](/upload/website/prouct_img/normal/GTX105986/GTX105986_40506_20180126_WB_B_w_23060120_124.webp "Anti-GABA B Receptor 2 antibody [N2C1], Internal used in Western Blot (WB). GTX105986")
![Anti-GABA B Receptor 2 antibody [N2C1], Internal used in Western Blot (WB). GTX105986](/upload/website/prouct_img/normal/GTX105986/GTX105986_40506_WB_w_23060120_928.webp "Anti-GABA B Receptor 2 antibody [N2C1], Internal used in Western Blot (WB). GTX105986")