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GABA Transporter 1 / GAT1 antibody

Anti-GABA Transporter 1 / GAT1 antibody used in IHC (Paraffin sections) (IHC-P). GTX133150
Anti-GABA Transporter 1 / GAT1 antibody used in Western Blot (WB). GTX133150
Anti-GABA Transporter 1 / GAT1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX133150
Anti-GABA Transporter 1 / GAT1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133150
Anti-GABA Transporter 1 / GAT1 antibody used in IHC (Paraffin sections) (IHC-P). GTX133150

Cat No. GTX133150

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

67 kDa. ( Note )

Positive Control

rat cerebellum

Predict Reactivity

Human, Bovine, Xenopus laevis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.53 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Recombinant protein encompassing a sequence within the center region of mouse GABA Transporter 1 / GAT1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886844

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

solute carrier family 6 (neurotransmitter transporter, GABA), member 1 , A730043E01 , GABATHG , GABATR , GAT-1 , Gabt , Gabt1 , Gat1 , XT-1 , Xtrp1

Cellular Localization

Cell membrane; Multi-pass membrane protein , Membrane; Multi-pass membrane protein

Background

Terminates the action of GABA by its high affinity sodium-dependent reuptake into presynaptic terminals.

Database

Research Area

DATA IMAGES

Anti-GABA Transporter 1 / GAT1 antibody used in IHC (Paraffin sections) (IHC-P). GTX133150

GTX133150 IHC-P Image

GABA Transporter 1 / GAT1 antibody detects GABA Transporter 1 / GAT1 protein at cell membrane and cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
GABA Transporter 1 / GAT1 antibody (GTX133150) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GABA Transporter 1 / GAT1 antibody used in Western Blot (WB). GTX133150

GTX133150 WB Image

Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with GABA Transporter 1 / GAT1 antibody (GTX133150) diluted at 1:500.

Anti-GABA Transporter 1 / GAT1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX133150

GTX133150 IHC-Fr Image

GABA Transporter 1 / GAT1 antibody detects GABA Transporter 1 / GAT1 protein by immunohistochemical analysis.
Samples: Frozen Sectioned adult mouse hippocampus.
Green: GABA Transporter 1 / GAT1 protein stained by GABA Transporter 1 / GAT1 antibody (GTX133150) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

Antigen Retrieval: Citrate buffer, pH 6.0, 10 min

Anti-GABA Transporter 1 / GAT1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133150

GTX133150 ICC/IF Image

GABA Transporter 1 / GAT1 antibody detects GABA Transporter 1 / GAT1 protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GABA Transporter 1 / GAT1 stained by GABA Transporter 1 / GAT1 antibody (GTX133150) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-GABA Transporter 1 / GAT1 antibody used in IHC (Paraffin sections) (IHC-P). GTX133150

GTX133150 IHC-P Image

GABA Transporter 1 / GAT1 antibody detects GABA Transporter 1 / GAT1 protein at cell membrane and cytoplasm in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
GABA Transporter 1 / GAT1 antibody (GTX133150) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169