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GABA antibody

Anti-GABA antibody used in IHC (Paraffin sections) (IHC-P). GTX125988
Anti-GABA antibody used in IHC (Frozen sections) (IHC-Fr). GTX125988
Anti-GABA antibody used in Dot blot (Dot). GTX125988
Anti-GABA antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125988

Cat. No. GTX125988

Reference 3
2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 ICC/IF, IHC-P, IHC-Fr, Dot, IHC

Reactivity

 Human, Mouse, Rat, Drosophila
Package
100 μl ($399),
25 μl ($169)
See all GABA products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Dot Assay dependent
IHC Assay dependent
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7), 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen used to generate this antibody corresponds to GABA

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11173015

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Research Area

DATA IMAGES

Anti-GABA antibody used in IHC (Paraffin sections) (IHC-P). GTX125988

GTX125988 IHC-P Image

GABA antibody detects GABA at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
GABA antibody (GTX125988) diluted at 1:400.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GABA antibody used in IHC (Frozen sections) (IHC-Fr). GTX125988

GTX125988 IHC-Fr Image

GABA antibody detects GABA by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: GABA stained by GABA antibody (GTX125988) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-GABA antibody used in Dot blot (Dot). GTX125988

GTX125988 Dot Image

Dot blot analysis of 100ng of GABA-conjugated BSA, using GABA Antibody (GTX125988) at 1:10000, 1:30000, and 1:90000. A: GABA-conjugated BSA. B: Normal BSA

Anti-GABA antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125988

GTX125988 ICC/IF Image

GABA antibody detects GABA at GABAergic neurons by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GABA stained by GABA antibody (GTX125988) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

Application Reference

Martirosian V et al. Cell Rep 2021; 35 (13) : 109302 Medulloblastoma uses GABA transaminase to survive in the cerebrospinal fluid microenvironment and promote leptomeningeal dissemination.
Wang D et al. Neurobiol Stress 2021; 14 : 100298 Lateral hypothalamus orexinergic inputs to lateral habenula modulate maladaptation after social defeat stress.
Application : IHC-Fr
Reactivity : Mouse
Chi H et al. Sci Rep 2020; 10 (1) : 13482 Cleavage of human tau at Asp421 inhibits hyperphosphorylated tau induced pathology in a Drosophila model.
Application : ICC/IF
Submit a Reference

REVIEW

GABA antibody Cat. No. GTX125988

Rating
( Average 4 based on 2 users reviews)
Application
IHC (Frozen sections)(IHC-Fr)
( Average 3 based on 1 users reviews)
Immunohistochemistry(IHC)
( Average 5 based on 1 users reviews)
Anti-GABA antibody used in Immunohistochemistry (IHC). GTX125988
Date : Anonymous submitted on 25-Jul-2018
Score :
Application Tested : IHC
Sample Species : Dm
Sample : Brain
Blocking : 5% goat serum in 0.3% PBST, 25°C, 1Hr
Fixation : 4% PFA
Primary Antibody : 1:100, 4°C, 16Hr
Anti-GABA antibody used in IHC (Frozen sections) (IHC-Fr). GTX125988
Date : Anonymous submitted on 20-Aug-2013
Score :
Application Tested : IHC-Fr
Sample Species : Ms
Sample : mouse brain
Blocking : 5% BSA, 25°C, 1Hr
Fixation : 4% PFA
Permeabilization /Antigen retrieval : 0.4 % Triton X-100 in PBS/10 mM sodium citrate
Primary Antibody : 1:2000, 4°C, 16Hr
Figure Note : DG, dentate gyrus; cc, corpus callosum; Cpu, caudate putamen (striatum); TH, thalamus.
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169