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GABA antibody

Anti-GABA antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125988
Anti-GABA antibody used in Dot blot (Dot). GTX125988
Anti-GABA antibody used in IHC (Paraffin sections) (IHC-P). GTX125988

Cat. No. GTX125988

Reference 10
2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 ICC/IF, IHC-P, IHC-Fr, Dot, IHC

Reactivity

 Species independent
Package
100 μl ($399),
25 μl ($169)
See all GABA products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Dot Assay dependent
IHC Assay dependent
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.71 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen used to generate this antibody corresponds to GABA

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11173015

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

Anti-GABA antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125988

GTX125988 ICC/IF Image

GABA antibody detects GABA protein at cell membrane by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GABA stained by GABA antibody (GTX125988) diluted at 1:250.
Red: Tau, a axon marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-GABA antibody used in Dot blot (Dot). GTX125988

GTX125988 Dot Image

Dot blot analysis of 100ng of GABA-conjugated BSA, using GABA Antibody (GTX125988) at 1:10000, 1:30000, and 1:90000. A: GABA-conjugated BSA. B: Normal BSA

Anti-GABA antibody used in IHC (Paraffin sections) (IHC-P). GTX125988

GTX125988 IHC-P Image

GABA antibody detects GABA at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
GABA antibody (GTX125988) diluted at 1:400.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Xiao S et al. Cereb Cortex 2023; 33 (20) : 10711-10721 Electroacupuncture alleviates the relapse of pain-related aversive memory by activating KOR and inhibiting GABAergic neurons in the insular cortex.
Guo Y et al. iScience 2023; 26 (9) : 107486 Ventrolateral periaqueductal gray GABAergic neurons promote arousal of sevoflurane anesthesia through cortico-midbrain circuit.
Mart?n-Su?rez S et al. Brain 2023; 146 (8) : 3416-3430 Blockage of STAT3 during epileptogenesis prevents GABAergic loss and imprinting of the epileptic state.
Cao F et al. Nan Fang Yi Ke Da Xue Xue Bao 2023; 43 (5) : 718-726 [Activation of GABAergic neurons in the zona incerta accelerates anesthesia induction with sevoflurane and propofol without affecting anesthesia maintenance or awakening in mice].
Wang Y et al. iScience 2023; 26 (1) : 105913 GABAergic neurons in the dorsomedial hypothalamus regulate states of consciousness in sevoflurane anesthesia.
Application : IHC-Fr
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Submit a Reference

REVIEW

GABA antibody Cat. No. GTX125988

Rating
( Average 4 based on 2 users reviews)
Application
IHC (Frozen sections)(IHC-Fr)
( Average 3 based on 1 users reviews)
Immunohistochemistry(IHC)
( Average 5 based on 1 users reviews)
Anti-GABA antibody used in Immunohistochemistry (IHC). GTX125988
Date : Anonymous submitted on 25-Jul-2018
Score :
Application Tested : IHC
Sample : Brain
Blocking : 5% goat serum in 0.3% PBST, 25°C, 1Hr
Fixation : 4% PFA
Primary Antibody : 1:100, 4°C, 16Hr
Anti-GABA antibody used in IHC (Frozen sections) (IHC-Fr). GTX125988
Date : Anonymous submitted on 20-Aug-2013
Score :
Application Tested : IHC-Fr
Sample : mouse brain
Blocking : 5% BSA, 25°C, 1Hr
Fixation : 4% PFA
Permeabilization /Antigen retrieval : 0.4 % Triton X-100 in PBS/10 mM sodium citrate
Primary Antibody : 1:2000, 4°C, 16Hr
Figure Note : DG, dentate gyrus; cc, corpus callosum; Cpu, caudate putamen (striatum); TH, thalamus.
Submit a Review
Application Reference
Xiao S et al. Cereb Cortex 2023; 33 (20):10711-10721 Electroacupuncture alleviates the relapse of pain-related aversive memory by activating KOR and inhibiting GABAergic neurons in the insular cortex.
Guo Y et al. iScience 2023; 26 (9):107486 Ventrolateral periaqueductal gray GABAergic neurons promote arousal of sevoflurane anesthesia through cortico-midbrain circuit.
Mart?n-Su?rez S et al. Brain 2023; 146 (8):3416-3430 Blockage of STAT3 during epileptogenesis prevents GABAergic loss and imprinting of the epileptic state.
Cao F et al. Nan Fang Yi Ke Da Xue Xue Bao 2023; 43 (5):718-726 [Activation of GABAergic neurons in the zona incerta accelerates anesthesia induction with sevoflurane and propofol without affecting anesthesia maintenance or awakening in mice].
Wang Y et al. iScience 2023; 26 (1):105913 GABAergic neurons in the dorsomedial hypothalamus regulate states of consciousness in sevoflurane anesthesia.
Application : IHC-Fr
Liu Y et al. Front Aging Neurosci 2022; 14:1036428 Endocannabinoid signaling regulates post-operative delirium through glutamatergic mediodorsal thalamus-prelimbic prefrontal cortical projection.
Application : IHC-Fr
Tanaka NK et al. J Neurosci 2021; 41 (47):9732-9741 A Sexually Dimorphic Olfactory Neuron Mediates Fixed Action Transition during Courtship Ritual in Drosophila melanogaster.
Application : IHC-P
Martirosian V et al. Cell Rep 2021; 35 (13):109302 Medulloblastoma uses GABA transaminase to survive in the cerebrospinal fluid microenvironment and promote leptomeningeal dissemination.
Wang D et al. Neurobiol Stress 2021; 14:100298 Lateral hypothalamus orexinergic inputs to lateral habenula modulate maladaptation after social defeat stress.
Application : IHC-Fr
Chi H et al. Sci Rep 2020; 10 (1):13482 Cleavage of human tau at Asp421 inhibits hyperphosphorylated tau induced pathology in a Drosophila model.
Application : ICC/IF
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169