GeneTex
United States (US)

GABA antibody

GTX125988_40744_20170503_IFA_R_18121410_646.jpg
GTX125988_40744_20151012_IHC-P_R_18121410_726.jpg
GTX125988_40744_20161027_IHC-Fr_18121410_226.jpg
GTX125988_40744_Dot_18121410_164.jpg

Cat No. GTX125988

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application ICC/IF, IHC-P, IHC-Fr, Dot, IHC
Reactivity Human, Mouse, Rat, Drosophila
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Dot Assay dependent
IHC Assay dependent
Not tested in other applications.

Positive Control

GABA-conjugated BSA , *mouse brain ,
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen used to generate this antibody corresponds to GABA

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
DATA IMAGES
GTX125988_40744_20170503_IFA_R_18121410_646.jpg

GTX125988 ICC/IF Image

GABA antibody detects GABA at GABAergic neurons by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GABA stained by GABA antibody (GTX125988) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX125988_40744_20151012_IHC-P_R_18121410_726.jpg

GTX125988 IHC-P Image

GABA antibody detects GABA protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
GABA antibody (GTX125988) diluted at 1:400.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX125988_40744_20161027_IHC-Fr_18121410_226.jpg

GTX125988 IHC-Fr Image

GABA antibody detects GABA by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: GABA stained by GABA antibody (GTX125988) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

GTX125988_40744_Dot_18121410_164.jpg

GTX125988 Dot Image

Dot blot analysis of 100ng of GABA-conjugated BSA, using GABA Antibody (GTX125988) at 1:10000, 1:30000, and 1:90000. A: GABA-conjugated BSA. B: Normal BSA

REVIEW

GABA antibody Cat No. GTX125988

Rating ( Average 4 based on 2 users reviews)
Application
IHC (Frozen sections)(IHC-Fr) ( Average 3 based on 1 users reviews)
Immunohistochemistry(IHC) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 25-Jul-2018
Score :
Application Tested : IHC
Sample Species : Dm
Sample : Brain
Blocking : 5% goat serum in 0.3% PBST, 25°C, 1Hr
Fixation : 4% PFA
Primary Antibody : 1:100, 4°C, overnightHr
Date : Anonymous submitted on 20-Aug-2013
Score :
Application Tested : IHC-Fr
Sample Species : Ms
Sample : mouse brain
Blocking : 5% BSA, 25°C, 1Hr
Fixation : 4% PFA
Permeabilization /Antigen retrieval : 0.4 % Triton X-100 in PBS/10 mM sodium citrate
Primary Antibody : 1:2000, 4°C, 16Hr
Figure Note : DG, dentate gyrus; cc, corpus callosum; Cpu, caudate putamen (striatum); TH, thalamus.
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169