APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:500-1:10000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
293T , Jurkat , Raji , NCI-H929 , A431 , HeLa , human GABARAP-transfected 293T
Predict Reactivity
Mouse, Rat, Rabbit, Bovine, Pig, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
1XPBS (pH7), 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human GABARAP. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2886075
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
GABA type A receptor-associated protein , ATG8A , GABARAP-a , MM46
Cellular Localization
Endomembrane system , Cytoplasm , cytoskeleton , Golgi apparatus membrane
Background
Gamma-aminobutyric acid A receptors [GABA(A) receptors] are ligand-gated chloride channels that mediate inhibitory neurotransmission. This gene encodes GABA(A) receptor-associated protein, which is highly positively charged in its N-terminus and shares sequence similarity with light chain-3 of microtubule-associated proteins 1A and 1B. This protein clusters neurotransmitter receptors by mediating interaction with the cytoskeleton. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX129710 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with GABARAP antibody (GTX129710) diluted at 1:1000.
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GTX129710 WB Image
GABARAP antibody detects GABARAP protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 whole cell lysate/extract of human GABARAP -transfected 293T cells
15 % SDS-PAGE
GABARAP antibody (GTX129710) dilution: 1:10000
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GTX129710 WB Image
GABARAP antibody detects GABARAP protein by western blot analysis.
A. 30 μg Jurkat whole cell lysate/extract
B. 30 μg Raji whole cell lysate/extract
C. 30 μg NCI-H929 whole cell lysate/extract
15 % SDS-PAGE
GABARAP antibody (GTX129710) dilution: 1:2000
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GTX129710 WB Image
GABARAP antibody detects GABARAP protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
15 % SDS-PAGE
GABARAP antibody (GTX129710) dilution: 1:2000
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GTX129710 ICC/IF Image
GABARAP antibody detects GABARAP protein at cytoplasm by immunofluorescent analysis.
Sample: U2OS cells were fixed in ice-cold MeOH for 5 min.
Green: GABARAP protein stained by GABARAP antibody (GTX129710) diluted at 1:500.
Blue: Hoechst 33342 staining.
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REFERENCE
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