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GABARAP antibody

Anti-GABARAP antibody used in Western Blot (WB). GTX129710
Anti-GABARAP antibody used in Western Blot (WB). GTX129710
Anti-GABARAP antibody used in Western Blot (WB). GTX129710
Anti-GABARAP antibody used in Western Blot (WB). GTX129710
Anti-GABARAP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129710

Cat No. GTX129710

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

14 kDa. ( Note )

Positive Control

293T , Jurkat , Raji , NCI-H929 , A431 , HeLa , human GABARAP-transfected 293T

Predict Reactivity

Mouse, Rat, Rabbit, Bovine, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human GABARAP. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

GABA type A receptor-associated protein , ATG8A , GABARAP-a , MM46

Cellular Localization

Endomembrane system , Cytoplasm , cytoskeleton , Golgi apparatus membrane

Background

Gamma-aminobutyric acid A receptors [GABA(A) receptors] are ligand-gated chloride channels that mediate inhibitory neurotransmission. This gene encodes GABA(A) receptor-associated protein, which is highly positively charged in its N-terminus and shares sequence similarity with light chain-3 of microtubule-associated proteins 1A and 1B. This protein clusters neurotransmitter receptors by mediating interaction with the cytoskeleton. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-GABARAP antibody used in Western Blot (WB). GTX129710

GTX129710 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with GABARAP antibody (GTX129710) diluted at 1:1000.

Anti-GABARAP antibody used in Western Blot (WB). GTX129710

GTX129710 WB Image

GABARAP antibody detects GABARAP protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 whole cell lysate/extract of human GABARAP -transfected 293T cells
15 % SDS-PAGE
GABARAP antibody (GTX129710) dilution: 1:10000

Anti-GABARAP antibody used in Western Blot (WB). GTX129710

GTX129710 WB Image

GABARAP antibody detects GABARAP protein by western blot analysis.
A. 30 μg Jurkat whole cell lysate/extract
B. 30 μg Raji whole cell lysate/extract
C. 30 μg NCI-H929 whole cell lysate/extract
15 % SDS-PAGE
GABARAP antibody (GTX129710) dilution: 1:2000

Anti-GABARAP antibody used in Western Blot (WB). GTX129710

GTX129710 WB Image

GABARAP antibody detects GABARAP protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
15 % SDS-PAGE
GABARAP antibody (GTX129710) dilution: 1:2000

Anti-GABARAP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129710

GTX129710 ICC/IF Image

GABARAP antibody detects GABARAP protein at cytoplasm by immunofluorescent analysis.
Sample: U2OS cells were fixed in ice-cold MeOH for 5 min.
Green: GABARAP protein stained by GABARAP antibody (GTX129710) diluted at 1:500.
Blue: Hoechst 33342 staining.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169