APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:500-1:10000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
293T , Jurkat , Raji , NCI-H929 , A431 , HeLa , human GABARAP-transfected 293T
Predict Reactivity
Mouse, Rat, Rabbit, Bovine, Pig, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human GABARAP. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
GABA type A receptor-associated protein , ATG8A , GABARAP-a , MM46
Cellular Localization
Endomembrane system , Cytoplasm , cytoskeleton , Golgi apparatus membrane
Background
Gamma-aminobutyric acid A receptors [GABA(A) receptors] are ligand-gated chloride channels that mediate inhibitory neurotransmission. This gene encodes GABA(A) receptor-associated protein, which is highly positively charged in its N-terminus and shares sequence similarity with light chain-3 of microtubule-associated proteins 1A and 1B. This protein clusters neurotransmitter receptors by mediating interaction with the cytoskeleton. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX129710 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with GABARAP antibody (GTX129710) diluted at 1:1000.
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GTX129710 WB Image
GABARAP antibody detects GABARAP protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 whole cell lysate/extract of human GABARAP -transfected 293T cells
15 % SDS-PAGE
GABARAP antibody (GTX129710) dilution: 1:10000
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GTX129710 WB Image
GABARAP antibody detects GABARAP protein by western blot analysis.
A. 30 μg Jurkat whole cell lysate/extract
B. 30 μg Raji whole cell lysate/extract
C. 30 μg NCI-H929 whole cell lysate/extract
15 % SDS-PAGE
GABARAP antibody (GTX129710) dilution: 1:2000
|
|
GTX129710 WB Image
GABARAP antibody detects GABARAP protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
15 % SDS-PAGE
GABARAP antibody (GTX129710) dilution: 1:2000
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GTX129710 ICC/IF Image
GABARAP antibody detects GABARAP protein at cytoplasm by immunofluorescent analysis.
Sample: U2OS cells were fixed in ice-cold MeOH for 5 min.
Green: GABARAP protein stained by GABARAP antibody (GTX129710) diluted at 1:500.
Blue: Hoechst 33342 staining.
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REFERENCE
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REVIEW
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