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GABARAP antibody

Anti-GABARAP antibody used in Western Blot (WB). GTX132657
Anti-GABARAP antibody used in Western Blot (WB). GTX132657
Anti-GABARAP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX132657

Cat No. GTX132657

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

14 kDa. ( Note )

Positive Control

293T , U87-MG , SK-N-SH , IMR32 , SK-N-AS

Predict Reactivity

Mouse, Rat, Rabbit, Bovine, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2.48 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human GABARAP. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886701

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

GABA type A receptor-associated protein , ATG8A , GABARAP-a , MM46

Cellular Localization

Endomembrane system , Cytoplasm , cytoskeleton , Golgi apparatus membrane

Background

Gamma-aminobutyric acid A receptors [GABA(A) receptors] are ligand-gated chloride channels that mediate inhibitory neurotransmission. This gene encodes GABA(A) receptor-associated protein, which is highly positively charged in its N-terminus and shares sequence similarity with light chain-3 of microtubule-associated proteins 1A and 1B. This protein clusters neurotransmitter receptors by mediating interaction with the cytoskeleton. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-GABARAP antibody used in Western Blot (WB). GTX132657

GTX132657 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with GABARAP antibody (GTX132657) diluted at 1:3000.

Anti-GABARAP antibody used in Western Blot (WB). GTX132657

GTX132657 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with GABARAP antibody (GTX132657) diluted at 1:1000.
The observed M.W. is based on the publication: PMID: 16303767 , PMID: 15187094

Anti-GABARAP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX132657

GTX132657 ICC/IF Image

GABARAP antibody detects GABARAP protein at cytoplasm by immunofluorescent analysis.
Sample: U-87 MG cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GABARAP protein stained by GABARAP antibody (GTX132657) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169