*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
293T , U87-MG , SK-N-SH , IMR32 , SK-N-AS
Mouse, Rat, Rabbit, Bovine, Pig, Rhesus Monkey(>80% identity)
1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
2.48 mg/ml (Please refer to the vial label for the specific concentration.)
Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human GABARAP. The exact sequence is proprietary.
Purified by antigen-affinity chromatography.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
GABA type A receptor-associated protein,ATG8A,GABARAP-a,MM46
Endomembrane system , Cytoplasm , cytoskeleton , Golgi apparatus membrane
Gamma-aminobutyric acid A receptors [GABA(A) receptors] are ligand-gated chloride channels that mediate inhibitory neurotransmission. This gene encodes GABA(A) receptor-associated protein, which is highly positively charged in its N-terminus and shares sequence similarity with light chain-3 of microtubule-associated proteins 1A and 1B. This protein clusters neurotransmitter receptors by mediating interaction with the cytoskeleton. [provided by RefSeq]
GTX132657 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with GABARAP antibody (GTX132657) diluted at 1:3000.
GTX132657 WB Image
Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with GABARAP antibody (GTX132657) diluted at 1:1000.
The observed M.W. is based on the publication: PMID: 16303767 and 15187094
GTX132657 ICC/IF Image
GABARAP antibody detects GABARAP protein at cytoplasm by immunofluorescent analysis.
Sample: U-87 MG cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GABARAP protein stained by GABARAP antibody (GTX132657) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.