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GABARAP antibody

Anti-GABARAP antibody used in Western Blot (WB). GTX132657
Anti-GABARAP antibody used in Western Blot (WB). GTX132657
Anti-GABARAP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX132657

Cat No. GTX132657

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

14 kDa. ( Note )

Positive Control

293T , U87-MG , SK-N-SH , IMR32 , SK-N-AS

Predict Reactivity

Mouse, Rat, Rabbit, Bovine, Pig, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2.48 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human GABARAP. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

GABA type A receptor-associated protein,ATG8A,GABARAP-a,MM46

Cellular Localization

Endomembrane system , Cytoplasm , cytoskeleton , Golgi apparatus membrane

Background

Gamma-aminobutyric acid A receptors [GABA(A) receptors] are ligand-gated chloride channels that mediate inhibitory neurotransmission. This gene encodes GABA(A) receptor-associated protein, which is highly positively charged in its N-terminus and shares sequence similarity with light chain-3 of microtubule-associated proteins 1A and 1B. This protein clusters neurotransmitter receptors by mediating interaction with the cytoskeleton. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-GABARAP antibody used in Western Blot (WB). GTX132657

GTX132657 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with GABARAP antibody (GTX132657) diluted at 1:3000.

Anti-GABARAP antibody used in Western Blot (WB). GTX132657

GTX132657 WB Image

Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with GABARAP antibody (GTX132657) diluted at 1:1000.
The observed M.W. is based on the publication: PMID: 16303767 and 15187094

Anti-GABARAP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX132657

GTX132657 ICC/IF Image

GABARAP antibody detects GABARAP protein at cytoplasm by immunofluorescent analysis.
Sample: U-87 MG cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GABARAP protein stained by GABARAP antibody (GTX132657) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

REFERENCE
REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169