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GABARAPL1 antibody

Anti-GABARAPL1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129277
Anti-GABARAPL1 antibody used in Western Blot (WB). GTX129277
Anti-GABARAPL1 antibody used in Western Blot (WB). GTX129277
Anti-GABARAPL1 antibody used in Western Blot (WB). GTX129277
Anti-GABARAPL1 antibody used in Western Blot (WB). GTX129277

Cat No. GTX129277

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

14 kDa. ( Note )

Positive Control

293T , HeLa , human GABARAPL1-transfected 293T

Predict Reactivity

Mouse, Rat, Bovine, Guinea pig, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human GABARAPL1 Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

GABA type A receptor associated protein like 1 , APG8-LIKE , APG8L , ATG8 , ATG8B , ATG8L , GEC1

Cellular Localization

Cytoplasm , Membrane , Endoplasmic reticulum , Golgi apparatus

Background

Increases cell-surface expression of kappa-type opioid receptor through facilitating anterograde intracellular trafficking of the receptor.

Database

Research Area

DATA IMAGES

Anti-GABARAPL1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129277

GTX129277 ICC/IF Image

GABARAPL1 antibody detects GABARAPL1 protein at autophagosome by immunofluorescent analysis.
Samples: HeLa cells mock (left) and treated with 50μM Chloroquine for 24 hr (right) were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GABARAPL1 protein stained by GABARAPL1 antibody (GTX129277) diluted at 1:1000.
Red: Phalloidin, a F-actin marker.

Anti-GABARAPL1 antibody used in Western Blot (WB). GTX129277

GTX129277 WB Image

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membranes were blotted with GABARAPL1 antibody (GTX129277) diluted at 1:500 and competitor's antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-GABARAPL1 antibody used in Western Blot (WB). GTX129277

GTX129277 WB Image

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membranes were blotted with GABARAPL1 antibody (GTX129277) diluted at 1:500 and competitor's antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-GABARAPL1 antibody used in Western Blot (WB). GTX129277

GTX129277 WB Image

GABARAPL1 antibody detects GABARAPL1 protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg whole cell lysate/extract of human GABARAPL1-transfected 293T cells
15 % SDS-PAGE
GABARAPL1 antibody (GTX129277) dilution: 1:1000

Anti-GABARAPL1 antibody used in Western Blot (WB). GTX129277

GTX129277 WB Image

GABARAPL1 antibody detects GABARAPL1 protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg HeLa whole cell lysate/extract
15 % SDS-PAGE
GABARAPL1 antibody (GTX129277) dilution: 1:500

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169