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GABARAPL1 antibody

Anti-GABARAPL1 antibody used in Western Blot (WB). GTX132664
Anti-GABARAPL1 antibody used in Western Blot (WB). GTX132664
Anti-GABARAPL1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX132664

Cat No. GTX132664

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

14 kDa. ( Note )

Positive Control

U87MG , SKNSH , mouse brain , rat brain

Predict Reactivity

Bovine, Guinea pig, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.3 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human GABARAPL1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

GABA type A receptor associated protein like 1 , APG8-LIKE , APG8L , ATG8 , ATG8B , ATG8L , GEC1

Cellular Localization

Cytoplasm , Membrane , Endoplasmic reticulum , Golgi apparatus

Background

Increases cell-surface expression of kappa-type opioid receptor through facilitating anterograde intracellular trafficking of the receptor.

Database

Research Area

DATA IMAGES

Anti-GABARAPL1 antibody used in Western Blot (WB). GTX132664

GTX132664 WB Image

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membranes were blotted with GABARAPL1 antibody (GTX132664) diluted at 1:500 and competitor's antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-GABARAPL1 antibody used in Western Blot (WB). GTX132664

GTX132664 WB Image

Various whole cell extracts (60 μg) and tissue extracts (15 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with GABARAPL1 antibody (GTX132664) diluted at 1:1000.

Anti-GABARAPL1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX132664

GTX132664 ICC/IF Image

GABARAPL1 antibody detects GABARAPL1 protein at autophagosome by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GABARAPL1 protein stained by GABARAPL1 antibody (GTX132664) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:50.
Blue: Hoechst 33342 staining.

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169