GeneTex
United States (US)

GABARAPL2 antibody

GTX102006_39596_20150612_WB_19011506_450.jpg
GTX102006_39596_20160720_IHC-P_M_19011506_787.jpg
GTX102006_39596_IHC_19011506_418.jpg

Cat No. GTX102006

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

14 kDa. ( Note )

Positive Control

Jurkat , Raji , NCI-H929

Predict Reactivity

Zebrafish, Bovine, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 40% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human GABARAPL2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

ATG8 antibody, ATG8C antibody, GATE-16 antibody, GATE16 antibody, GEF2 antibody, GABARAPL2 antibody, MAP1 light chain 3-related protein antibody, golgi-associated ATPase enhancer of 16 kDa antibody, MAP1 light chain 3 related protein antibody, general protein transport factor p16 antibody, ganglioside expression factor 2 antibody, gamma-aminobutyric acid receptor-associated protein-like 2 antibody, GABA(A) receptor-associated protein-like 2 antibody

Background

Involved in intra-Golgi traffic. Modulates intra-Golgi transport through coupling between NSF activity and SNAREs activation. It first stimulates the ATPase activity of NSF which in turn stimulates the association with GOSR1.

Database

Research Area

DATA IMAGES
GTX102006_39596_20150612_WB_19011506_450.jpg

GTX102006 WB Image

GABARAPL2 antibody detects GABARAPL2 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with GABARAPL2 antibody (GTX102006) diluted at a dilution of 1:1500.

GTX102006_39596_20160720_IHC-P_M_19011506_787.jpg

GTX102006 IHC-P Image

GABARAPL2 antibody detects GABARAPL2 protein at cytoplasm in mouse cervix by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cervix.
GABARAPL2 antibody (GTX102006) diluted at 1:200.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX102006_39596_IHC_19011506_418.jpg

GTX102006 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SAS xenograft, using GABARAPL2(GTX102006) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
REVIEW

GABARAPL2 antibody Cat No. GTX102006

Rating ( Average 4 based on 1 users reviews)
Application
Western Blot(WB) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 30-Aug-2016
Score :
Application Tested : WB
Sample Species : Rat
Sample : Rat brain tissue
Amount used : 25μg
Blocking : 5% milk in TBST, 25°C, 1Hr
Primary Antibody : 1:100, 4°C, overnightHr
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf