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GABARAPL2 antibody

Anti-GABARAPL2 antibody used in Western Blot (WB). GTX132666
Anti-GABARAPL2 antibody used in Western Blot (WB). GTX132666
Anti-GABARAPL2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX132666

Cat No. GTX132666

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

14 kDa. ( Note )

Positive Control

HeLa , GABARAPL2 shRNA-transfected HeLa , SK-N-AS , mouse brain , rat brain

Predict Reactivity

Zebrafish, Bovine, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.43 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human GABARAPL2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

GABA type A receptor associated protein like 2,ATG8,ATG8C,GATE-16,GATE16,GEF-2,GEF2

Cellular Localization

Golgi apparatus

Database

Research Area

DATA IMAGES
Anti-GABARAPL2 antibody used in Western Blot (WB). GTX132666

GTX132666 WB Image

Non-transfected (–) and transfected (+) HeLa whole cell extracts (50 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with GABARAPL2 antibody (GTX132666) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-GABARAPL2 antibody used in Western Blot (WB). GTX132666

GTX132666 WB Image

Various tissue extracts (15 µg) and whole cell extracts (40 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with GABARAPL2 antibody (GTX132666) diluted at 1:1000.

Anti-GABARAPL2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX132666

GTX132666 ICC/IF Image

GABARAPL2 antibody detects GABARAPL2 protein at autophagosome  by immunofluorescent analysis.
Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GABARAPL2 stained by GABARAPL2 antibody (GTX132666) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar= 10 μm.

REFERENCE
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169