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GABPA antibody [8C1B10]

Anti-GABPA antibody [8C1B10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83195
Anti-GABPA antibody [8C1B10] used in Western Blot (WB). GTX83195
Anti-GABPA antibody [8C1B10] used in Western Blot (WB). GTX83195

Cat. No. GTX83195

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 8C1B10

Isotype

 IgG1

Application

 WB, ICC/IF, IP, ELISA

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all GABPA products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
ICC/IF 1/200 - 1/1000
IP Assay dependent
ELISA 1/10000
Not tested in other applications.

Calculated MW

51 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.03% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified recombinant fragment of human GABPA (aa120-190) expressed in E. Coli.

Purification

Unpurified

Conjugation

Unconjugated

RRID

AB_11165404

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

GA binding protein transcription factor subunit alpha , E4TF1-60 , E4TF1A , NFT2 , NRF2 , NRF2A , RCH04A07

Cellular Localization

Nucleus

Background

This gene encodes one of three GA-binding protein transcription factor subunits which functions as a DNA-binding subunit. Since this subunit shares identity with a subunit encoding the nuclear respiratory factor 2 gene, it is likely involved in activation of cytochrome oxidase expression and nuclear control of mitochondrial function. This subunit also shares identity with a subunit constituting the transcription factor E4TF1, responsible for expression of the adenovirus E4 gene. Because of its chromosomal localization and ability to form heterodimers with other polypeptides, this gene may play a role in the Down Syndrome phenotype. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Oct 2010]

Database

Research Area

DATA IMAGES

Anti-GABPA antibody [8C1B10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83195

GTX83195 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX83195 GABPA antibody [8C1B10].
Green : GABPA
Blue: DRAQ5 fluorescent DNA dye
Red: Actin filaments

Anti-GABPA antibody [8C1B10] used in Western Blot (WB). GTX83195

GTX83195 WB Image

WB analysis of HEK293T cells transfected with the pCMV6-ENTRY control (1) and pCMV6-ENTRY GABPA cDNA (2) using GTX83195 GABPA antibody [8C1B10].

Anti-GABPA antibody [8C1B10] used in Western Blot (WB). GTX83195

GTX83195 WB Image

WB analysis of HeLa (1), A549 (2), MCF-7 (3), NIH3T3 (4) and SMMC-7721 (5) cell lysate using GTX83195 GABPA antibody [8C1B10].

REFERENCE

Application Reference

Zheng X et al. Exp Cell Res 2017; (Epub) A Notch-independent mechanism contributes to the induction of Hes1 gene expression in response to hypoxia in P19 cells.
Application : WB, IP
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399