Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GABPA antibody [N2C2], Internal (GTX102572) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GABPA antibody [N2C2], Internal detects GABPA protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lymph node.
GABPA stained by GABPA antibody [N2C2], Internal (GTX102572) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GABPA antibody [N2C2], Internal detects GABPA protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat spleen.
GABPA stained by GABPA antibody [N2C2], Internal (GTX102572) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GABPA antibody [N2C2], Internal detects GABPA protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GABPA protein stained by GABPA antibody [N2C2], Internal (GTX102572) diluted at 1:1000.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Immunoprecipitation of GABPA protein from 293T whole cell extracts using 5 μg of GABPA antibody [N2C2], Internal (GTX102572).
Western blot analysis was performed using GABPA antibody [N2C2], Internal (GTX102572).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.