*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
293T , A431 , HeLa , HepG2
Mouse, Rat, Bovine, Chicken, Xenopus laevis, Rhesus Monkey(>80% identity)
1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
0.62 mg/ml (Please refer to the vial label for the specific concentration.)
Recombinant protein encompassing a sequence within the center region of human GABPA. The exact sequence is proprietary.
Purified by antigen-affinity chromatography.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Ga Binding Protein Transcription Factor Subunit Alpha,E4Tf1-60,E4Tf1A,Nft2,Nrf2,Nrf2A,Rch04A07,Gabpa
This gene encodes one of three GA-binding protein transcription factor subunits which functions as a DNA-binding subunit. Since this subunit shares identity with a subunit encoding the nuclear respiratory factor 2 gene, it is likely involved in activation of cytochrome oxidase expression and nuclear control of mitochondrial function. This subunit also shares identity with a subunit constituting the transcription factor E4TF1, responsible for expression of the adenovirus E4 gene. Because of its chromosomal localization and ability to form heterodimers with other polypeptides, this gene may play a role in the Down Syndrome phenotype. [provided by RefSeq]
GTX102572 IP Image
Immunoprecipitation of GABPA protein from 293T whole cell extracts using 5 μg of GABPA antibody [N2C2], Internal (GTX102572).
Western blot analysis was performed using GABPA antibody [N2C2], Internal (GTX102572).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
GTX102572 WB Image
GABPA antibody detects GABPA protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GABPA antibody (GTX102572) diluted at a dilution of 1:1000.
GTX102572 ICC/IF Image
GABPA antibody [N2C2], Internal detects GABPA protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GABPA protein stained by GABPA antibody [N2C2], Internal (GTX102572) diluted at 1:1000.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.