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GABPA antibody [N2C2], Internal

Anti-GABPA antibody [N2C2], Internal used in Immunoprecipitation (IP). GTX102572
Anti-GABPA antibody [N2C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102572
Anti-GABPA antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX102572
Anti-GABPA antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX102572
Anti-GABPA antibody [N2C2], Internal used in Western Blot (WB). GTX102572

Cat. No. GTX102572

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all GABPA products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P Assay dependent
IP 1:100-1:500
Not tested in other applications.

Calculated MW

51 kDa. ( Note )

Predict Reactivity

Bovine, Chicken, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.33 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human GABPA. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1240875

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

GA binding protein transcription factor subunit alpha , E4TF1-60 , E4TF1A , NFT2 , NRF2 , NRF2A , RCH04A07

Cellular Localization

Nucleus

Background

This gene encodes one of three GA-binding protein transcription factor subunits which functions as a DNA-binding subunit. Since this subunit shares identity with a subunit encoding the nuclear respiratory factor 2 gene, it is likely involved in activation of cytochrome oxidase expression and nuclear control of mitochondrial function. This subunit also shares identity with a subunit constituting the transcription factor E4TF1, responsible for expression of the adenovirus E4 gene. Because of its chromosomal localization and ability to form heterodimers with other polypeptides, this gene may play a role in the Down Syndrome phenotype. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-GABPA antibody [N2C2], Internal used in Immunoprecipitation (IP). GTX102572

GTX102572 IP Image

Immunoprecipitation of GABPA protein from 293T whole cell extracts using 5 μg of GABPA antibody [N2C2], Internal (GTX102572).
Western blot analysis was performed using GABPA antibody [N2C2], Internal (GTX102572).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-GABPA antibody [N2C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102572

GTX102572 ICC/IF Image

GABPA antibody [N2C2], Internal detects GABPA protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GABPA protein stained by GABPA antibody [N2C2], Internal (GTX102572) diluted at 1:1000.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-GABPA antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX102572

GTX102572 IHC-P Image

GABPA antibody [N2C2], Internal detects GABPA protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat spleen.
GABPA stained by GABPA antibody [N2C2], Internal (GTX102572) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GABPA antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX102572

GTX102572 IHC-P Image

GABPA antibody [N2C2], Internal detects GABPA protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lymph node.
GABPA stained by GABPA antibody [N2C2], Internal (GTX102572) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GABPA antibody [N2C2], Internal used in Western Blot (WB). GTX102572

GTX102572 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GABPA antibody [N2C2], Internal (GTX102572) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169