GeneTex
United States (US)

GABPA antibody [N2C2], Internal

GTX102572_42004_20151016_IP_19011506_511.jpg
GTX102572_42004_20150226_WB_19011506_457.jpg
GTX102572_42004_20150525_IFA_19011506_992.jpg

Cat No. GTX102572

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IP
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

51 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

Predict Reactivity

Mouse, Rat, Bovine, Chicken, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.62 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human GABPA. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Ga Binding Protein Transcription Factor Subunit Alpha,E4Tf1-60,E4Tf1A,Nft2,Nrf2,Nrf2A,Rch04A07,Gabpa

Cellular Localization

Nucleus

Background

This gene encodes one of three GA-binding protein transcription factor subunits which functions as a DNA-binding subunit. Since this subunit shares identity with a subunit encoding the nuclear respiratory factor 2 gene, it is likely involved in activation of cytochrome oxidase expression and nuclear control of mitochondrial function. This subunit also shares identity with a subunit constituting the transcription factor E4TF1, responsible for expression of the adenovirus E4 gene. Because of its chromosomal localization and ability to form heterodimers with other polypeptides, this gene may play a role in the Down Syndrome phenotype. [provided by RefSeq]

Database

DATA IMAGES
GTX102572_42004_20151016_IP_19011506_511.jpg

GTX102572 IP Image

Immunoprecipitation of GABPA protein from 293T whole cell extracts using 5 μg of GABPA antibody [N2C2], Internal (GTX102572).
Western blot analysis was performed using GABPA antibody [N2C2], Internal (GTX102572).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX102572_42004_20150226_WB_19011506_457.jpg

GTX102572 WB Image

GABPA antibody detects GABPA protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GABPA antibody (GTX102572) diluted at a dilution of 1:1000.

GTX102572_42004_20150525_IFA_19011506_992.jpg

GTX102572 ICC/IF Image

GABPA antibody [N2C2], Internal detects GABPA protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GABPA protein stained by GABPA antibody [N2C2], Internal (GTX102572) diluted at 1:1000.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169