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GABPB1 antibody [N1C3]

Anti-GABPB1 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX103464
Anti-GABPB1 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103464
Anti-GABPB1 antibody [N1C3] used in Western Blot (WB). GTX103464

Cat No. GTX103464

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , Molt-4 , Raji

Predict Reactivity

Mouse, Bovine, Chicken, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.63 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human GABPB1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

GA binding protein transcription factor subunit beta 1 , BABPB2 , E4TF1 , E4TF1-47 , E4TF1-53 , E4TF1B , GABPB , GABPB-1 , GABPB2 , NRF2B1 , NRF2B2

Cellular Localization

Nucleus

Background

This gene encodes the GA-binding protein transcription factor, beta subunit. This protein forms a tetrameric complex with the alpha subunit, and stimulates transcription of target genes. The encoded protein may be involved in activation of cytochrome oxidase expression and nuclear control of mitochondrial function. The crystal structure of a similar protein in mouse has been resolved as a ternary protein complex. Multiple transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq]

Database

DATA IMAGES
Anti-GABPB1 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX103464

GTX103464 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 Xenograft, using GABP-beta1(GTX103464) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GABPB1 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103464

GTX103464 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using GABPB1(GTX103464) antibody at 1:500 dilution.

Anti-GABPB1 antibody [N1C3] used in Western Blot (WB). GTX103464

GTX103464 WB Image

Sample (30 ug of whole cell lysate)
A: Hela
10% SDS PAGE
GTX103464 diluted at 1:5000

REFERENCE
REVIEW
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169