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GABPB1 antibody [N1C3]

Anti-GABPB1 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX103464
Anti-GABPB1 antibody [N1C3] used in Western Blot (WB). GTX103464
Anti-GABPB1 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103464

Cat. No. GTX103464

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all GABPB1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

Positive Control

HeLa

Predict Reactivity

Mouse, Bovine, Chicken, Xenopus laevis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.63 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human GABPB1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037029

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

GA binding protein transcription factor subunit beta 1 , BABPB2 , E4TF1 , E4TF1-47 , E4TF1-53 , E4TF1B , GABPB , GABPB-1 , GABPB2 , NRF2B1 , NRF2B2

Cellular Localization

Nucleus

Background

This gene encodes the GA-binding protein transcription factor, beta subunit. This protein forms a tetrameric complex with the alpha subunit, and stimulates transcription of target genes. The encoded protein may be involved in activation of cytochrome oxidase expression and nuclear control of mitochondrial function. The crystal structure of a similar protein in mouse has been resolved as a ternary protein complex. Multiple transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-GABPB1 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX103464

GTX103464 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 Xenograft, using GABP-beta1(GTX103464) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GABPB1 antibody [N1C3] used in Western Blot (WB). GTX103464

GTX103464 WB Image

Sample (30 ug of whole cell lysate)
A: Hela
10% SDS PAGE
GTX103464 diluted at 1:5000

Anti-GABPB1 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103464

GTX103464 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using GABPB1(GTX103464) antibody at 1:500 dilution.

REFERENCE

Application Reference

Ihashi S et al. Life Sci Alliance 2023; 6 (11) Incomplete activation of Alyref and Gabpb1 leads to preimplantation arrest in cloned mouse embryos.
Chen S.C. et al. Journal of Oncology 2018; (Epub) Knockdown of GA-binding protein subunit β1 inhibits cell proliferation via p21 induction in renal cell carcinoma.
Application : WB
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169