GeneTex
United States (US)

GAD65 antibody [144]

Cat No. GTX80325

Host Mouse
Clonality Monoclonal
Clone Name 144
Isotype IgG1
Application WB, FACS, IP, IHC
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

Recommended Starting Dilutions:
For WB: Use at 1μg/mL
Optimal dilutions should be determined experimentally by the researcher.

Calculated MW

65 kDa. ( Note )

Specificity/Sensitivity

GAD65 (human, mouse and rat, other specificities not tested)
PROPERTIES

Form

Liquid

Buffer

PBS + 0.1% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide corresponding to human GAD65 amino acids 4-22, coupled to KLH

Purification

Affinity purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

65 kDa glutamic acid decarboxylase antibody, DCE 2 antibody, DCE2 antibody, GAD 2 antibody, GAD 65 antibody, GAD-2 antibody, GAD-65 antibody, GAD2 antibody, Glutamate Decarboxylase 2 (pancreatic islets and brain 65kDa) antibody, Glutamate Decarboxylase 2 antibody, Glutamate Decarboxylase 65 antibody, Glutamate decarboxylase 65 kDa isoform antibody, Glutamic Acid Decarboxylase 2 antibody, Glutamic Acid Decarboxylase 65 antibody,

Cellular Localization

Associated to cytoplasmic vesicles. In neurons, cytosolic leaflet of Golgi membranes and presynaptic clusters.

Background

This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq]

Database

Research Area

Package List Price ($)
$ 319