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GAD65 antibody [C2C3], C-term

Anti-GAD65 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100281
Anti-GAD65 antibody [C2C3], C-term used in IHC (Frozen sections) (IHC-Fr). GTX100281
Anti-GAD65 antibody [C2C3], C-term used in Western Blot (WB). GTX100281
Anti-GAD65 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100281

Cat No. GTX100281

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

65 kDa. ( Note )

Positive Control

Mouse brain , rat brain

Predict Reactivity

Human, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human GAD65. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1240877

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

glutamate decarboxylase 2 , GAD65

Cellular Localization

Cytoplasm , cytosol , Cytoplasmic vesicle , Cell junction , synapse , presynaptic cell membrane , Golgi apparatus membrane

Background

This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-GAD65 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100281

GTX100281 IHC-P Image

GAD65 antibody [C2C3], C-term detects GAD2 protein at cytosol on RT2 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded RT2 xenograft.
GAD65 antibody [C2C3], C-term (GTX100281) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-GAD65 antibody [C2C3], C-term used in IHC (Frozen sections) (IHC-Fr). GTX100281

GTX100281 IHC-Fr Image

GAD65 antibody [C2C3], C-term detects GAD65 protein by immunohistochemical analysis.
Sample: Frozen sectioned adult mouse retina.
Green: GAD65 protein stained by GAD65 antibody [C2C3], C-term (GTX100281) diluted at 1:250.
Red: Protein kinase C alpha staining.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-GAD65 antibody [C2C3], C-term used in Western Blot (WB). GTX100281

GTX100281 WB Image

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GAD65 antibody [C2C3], C-term (GTX100281) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GAD65 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100281

GTX100281 ICC/IF Image

GAD65 antibody [C2C3], C-term detects GAD65 protein expression by immunofluorescent analysis.
Sample: Cultured Rat E18 primary cortical neuron, DIV 8. Cells were fixed in 4% paraformaldehyde at RT for 15 min.
Red: GAD65 protein stained by GAD65 antibody [C2C3], C-term (GTX100281) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920)..

REFERENCE

There are currently no references for GAD65 antibody [C2C3], C-term (GTX100281). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for GAD65 antibody [C2C3], C-term (GTX100281). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169