Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GAD65 antibody [C2C3], C-term (GTX100281) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GAD65 antibody [C2C3], C-term (GTX100281) diluted at 1:100000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GAD65 antibody [C2C3], C-term detects GAD65 protein expression by immunofluorescent analysis.
Sample: Cultured Rat E18 primary cortical neuron, DIV 8. Cells were fixed in 4% paraformaldehyde at RT for 15 min.
Red: GAD65 protein stained by GAD65 antibody [C2C3], C-term (GTX100281) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920)..

GAD65 antibody [C2C3], C-term detects GAD2 protein at cytosol on RT2 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded RT2 xenograft.
GAD65 antibody [C2C3], C-term (GTX100281) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GAD65 antibody [C2C3], C-term detects GAD65 protein by immunohistochemical analysis.
Sample: Frozen sectioned adult mouse retina.
Green: GAD65 protein stained by GAD65 antibody [C2C3], C-term (GTX100281) diluted at 1:250.
Red: Protein kinase C alpha staining.
Blue: Fluoroshield with DAPI (GTX30920).