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GAD65 antibody [C2C3], C-term

Overexpression
Anti-GAD65 antibody [C2C3], C-term used in IHC (Frozen sections) (IHC-Fr). GTX100281
Anti-GAD65 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100281
Anti-GAD65 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100281
Anti-GAD65 antibody [C2C3], C-term used in Western Blot (WB). GTX100281
Anti-GAD65 antibody [C2C3], C-term used in Western Blot (WB). GTX100281

Cat. No. GTX100281

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all GAD65 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

65 kDa. ( Note )

Positive Control

mouse brain , rat brain

Product Note

This antibody is specific for GAD65 protein, and it does not cross-react with GAD67 protein.

Predict Reactivity

Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human GAD65. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1240877

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glutamate decarboxylase 2 , GAD65

Cellular Localization

Cytoplasm , cytosol , Cytoplasmic vesicle , Cell junction , synapse , presynaptic cell membrane , Golgi apparatus membrane

Background

This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-GAD65 antibody [C2C3], C-term used in IHC (Frozen sections) (IHC-Fr). GTX100281

GTX100281 IHC-Fr Image

GAD65 antibody [C2C3], C-term detects GAD65 protein by immunohistochemical analysis.
Sample: Frozen sectioned adult mouse retina.
Green: GAD65 protein stained by GAD65 antibody [C2C3], C-term (GTX100281) diluted at 1:250.
Red: Protein kinase C alpha staining.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-GAD65 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100281

GTX100281 IHC-P Image

GAD65 antibody [C2C3], C-term detects GAD2 protein at cytosol on RT2 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded RT2 xenograft.
GAD65 antibody [C2C3], C-term (GTX100281) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-GAD65 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100281

GTX100281 ICC/IF Image

GAD65 antibody [C2C3], C-term detects GAD65 protein expression by immunofluorescent analysis.
Sample: Cultured Rat E18 primary cortical neuron, DIV 8. Cells were fixed in 4% paraformaldehyde at RT for 15 min.
Red: GAD65 protein stained by GAD65 antibody [C2C3], C-term (GTX100281) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920)..
Anti-GAD65 antibody [C2C3], C-term used in Western Blot (WB). GTX100281

GTX100281 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GAD65 antibody [C2C3], C-term (GTX100281) diluted at 1:100000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GAD65 antibody [C2C3], C-term used in Western Blot (WB). GTX100281

GTX100281 WB Image

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GAD65 antibody [C2C3], C-term (GTX100281) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169