APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 0.1-0.2μg/ml for 30 min at RT |
| Assay dependent |
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Note :
IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
|
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
10mM PBS, 0.05% BSA
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant human GAD2 (GAD65) protein fragment (around aa 6-99) (exact sequence is proprietary)
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
GAD2 , GAD65 , glutamate decarboxylase 2
Cellular Localization
Cytoplasm,Cytosol,Cytoplasmic vesicle,Cell junction,Synapse,Presynaptic cell membrane,Golgi apparatus membrane
Background
This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Oct 2008]
Database
Research Area
DATA IMAGES
![Anti-GAD65 antibody [GAD2/1960] used in Protein Array (Protein Array). GTX17966](/templates/default/js/lightbox/images/loading.gif "Anti-GAD65 antibody [GAD2/1960] used in Protein Array (Protein Array). GTX17966")
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GTX17966 Protein Array Image
Analysis of Protein Array containing more than 19,000 full-length human proteins using GAD2 (GAD65) Mouse Monoclonal Antibody (GAD2/1960). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target if the Monoclonal Antibody has an S-score of at least 2.5. For example, if a Monoclonal Antibody binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Monoclonal Antibody to protein X is equal to 29.
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GTX17966 IHC-P Image
IHC-P analysis of human pancreas tissue using GTX17966 GAD65 antibody [GAD2/1960].
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REFERENCE
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REVIEW
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![Anti-GAD65 antibody [GAD2/1960] used in Protein Array (Protein Array). GTX17966](/upload/website/prouct_img/small/GTX17966/GTX17966_20200115_Protein-Array_2031_w_23060620_116.webp "Anti-GAD65 antibody [GAD2/1960] used in Protein Array (Protein Array). GTX17966")
![Anti-GAD65 antibody [GAD2/1960] used in IHC (Paraffin sections) (IHC-P). GTX17966](/upload/website/prouct_img/small/GTX17966/GTX17966_20200115_IHC-P_873_w_23060620_755.webp "Anti-GAD65 antibody [GAD2/1960] used in IHC (Paraffin sections) (IHC-P). GTX17966")
![Anti-GAD65 antibody [GAD2/1960] used in Protein Array (Protein Array). GTX17966](/upload/website/prouct_img/normal/GTX17966/GTX17966_20200115_Protein-Array_2031_w_23060620_116.webp "Anti-GAD65 antibody [GAD2/1960] used in Protein Array (Protein Array). GTX17966")
![Anti-GAD65 antibody [GAD2/1960] used in IHC (Paraffin sections) (IHC-P). GTX17966](/upload/website/prouct_img/normal/GTX17966/GTX17966_20200115_IHC-P_873_w_23060620_755.webp "Anti-GAD65 antibody [GAD2/1960] used in IHC (Paraffin sections) (IHC-P). GTX17966")