GeneTex
United States (US)

GAD65 antibody

GTX113192_40086_IHC_R_18121410_298.jpg
GTX113192_40086_20180119_WB_M_R_18121410_946.jpg
GTX113192_40086_IFA_18121410_851.jpg
GTX113192_40086_20180613_ICC_IF_R_18121410_804.jpg
GTX113192_43089_20180105_WB_M_R_19011519_898.jpg

Cat No. GTX113192

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

65 kDa. ( Note )

Positive Control

mouse brain , rat brain

Predict Reactivity

Zebrafish, Bovine, Dog, Pig, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.93mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human GAD65. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

GAD65 antibody, MGC161605 antibody, MGC161607 antibody, GAD2 antibody, 65 kDa glutamic acid decarboxylase antibody, glutamate decarboxylase 65 kDa isoform antibody, GAD-65 antibody, glutamate decarboxylase 2 antibody, Glutamate decarboxylase-2 (pancreas) antibody, glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa) antibody

Cellular Localization

Cytoplasm , cytosol , Cytoplasmic vesicle , Cell junction , synapse , presynaptic cell membrane , Golgi apparatus membrane

Background

This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX113192_40086_IHC_R_18121410_298.jpg

GTX113192 IHC-P Image

GAD65 antibody detects GAD2 protein at cytosol on RT2 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded RT2 xenograft.
GAD65 antibody (GTX113192) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX113192_40086_20180119_WB_M_R_18121410_946.jpg

GTX113192 WB Image

Various tissue extracts (50 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GAD65 antibody (GTX113192) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX113192_40086_IFA_18121410_851.jpg

GTX113192 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using GAD65(GTX113192) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2500.

GTX113192_40086_20180613_ICC_IF_R_18121410_804.jpg

GTX113192 ICC/IF Image

GAD65 antibody detects GAD65 protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GAD65 stained by GAD65 antibody (GTX113192) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX113192_43089_20180105_WB_M_R_19011519_898.jpg

GTX113192 WB Image

Various tissue extracts (50 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GAD65 antibody (GTX113192) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169