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GAD65 antibody

Anti-GAD65 antibody used in IHC (Frozen sections) (IHC-Fr). GTX133096
Anti-GAD65 antibody used in Western Blot (WB). GTX133096

Cat. No. GTX133096

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IHC-Fr

Reactivity

 Human, Rat
Package
100 μl ($399),
25 μl ($169)
See all GAD65 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
IHC-P Assay dependent
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

65 kDa. ( Note )

Positive Control

APRE-19

Predict Reactivity

Mouse, Dog, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.22 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of mouse GAD65. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886823

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glutamic acid decarboxylase 2 , 6330404F12Rik , GAD(65) , GAD65 , Gad-2

Cellular Localization

Cytoplasm , cytosol , Cytoplasmic vesicle , Cell junction , synapse , presynaptic cell membrane; Lipid-anchor , Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side , Cytoplasm , cytosol , Cytoplasmic vesicle , Cell junction , synapse

Background

Catalyzes the production of GABA.

Database

Research Area

DATA IMAGES

Anti-GAD65 antibody used in IHC (Frozen sections) (IHC-Fr). GTX133096

GTX133096 IHC-Fr Image

GAD65 antibody detects GAD65 protein expression by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: GAD65 protein stained by GAD65 antibody (GTX133096) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-GAD65 antibody used in Western Blot (WB). GTX133096

GTX133096 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with GAD65 antibody (GTX133096) diluted at 1:5000.

REFERENCE

Application Reference

Barzroodi Pour M et al. Life Sci 2019; 232 : 116667 The effect of exercise on GABA signaling pathway in the model of chemically induced seizures.
Application : IHC-P
Reactivity : Rat
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169