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GAD65 antibody

Anti-GAD65 antibody used in IHC (Paraffin sections) (IHC-P). GTX133156
Anti-GAD65 antibody used in Western Blot (WB). GTX133156

Cat No. GTX133156

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

65 kDa. ( Note )

Positive Control

mouse brain , rat brain

Predict Reactivity

Human, Bovine, Dog, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.34 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of mouse GAD65. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Glutamic Acid Decarboxylase 2,6330404F12Rik,Gad(65),Gad65,Gad-2,Gad2

Cellular Localization

Cytoplasm , cytosol , Cytoplasmic vesicle , Cell junction , synapse , presynaptic cell membrane; Lipid-anchor , Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side , Cytoplasm , cytosol , Cytoplasmic vesicle , Cell junction , synapse

Background

Catalyzes the production of GABA.

Database

Research Area

DATA IMAGES
Anti-GAD65 antibody used in IHC (Paraffin sections) (IHC-P). GTX133156

GTX133156 IHC-P Image

GAD65 antibody detects GAD65 protein expression by immunohistochemical analysis.
Sample: Paraffin-Embedded adult mouse retina.
Green: GAD65 protein stained by GAD65 antibody (GTX133156) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GAD65 antibody used in Western Blot (WB). GTX133156

GTX133156 WB Image

Various tissue extracts (50 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GAD65 antibody (GTX133156) diluted at 1:1000.

REFERENCE
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169