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GAD65 antibody

Anti-GAD65 antibody used in IHC (Paraffin sections) (IHC-P). GTX32615
Anti-GAD65 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32615
Anti-GAD65 antibody used in Western Blot (WB). GTX32615

Cat No. GTX32615

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Rat
Package
100 μl ($329)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
ICC/IF 1:10 - 1:100
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

65 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 401-585 of human GAD2 (NP_001127838.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

glutamate decarboxylase 2 , GAD65

Cellular Localization

Cytoplasm, cytosol,Cytoplasmic vesicle,Golgi apparatus membrane

Background

This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Oct 2008]

Database

Research Area

DATA IMAGES
Anti-GAD65 antibody used in IHC (Paraffin sections) (IHC-P). GTX32615

GTX32615 IHC-P Image

IHC-P analysis of rat brain tissue using GTX32615 GAD65 antibody.
Dilution : 1:100

Anti-GAD65 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32615

GTX32615 ICC/IF Image

ICC/IF analysis of A549 cell using GTX32615 GAD65 antibody.
Blue : DAPI

Anti-GAD65 antibody used in Western Blot (WB). GTX32615

GTX32615 WB Image

WB analysis of various samples using GTX32615 GAD65 antibody.
Dilution : 1:1000
Loading : 25μg per lane

REFERENCE
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REVIEW
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Package List Price ($)
$ 329