GeneTex

  • Country / Location Selection

United States (US)

GAD67 antibody [GAD1/2391]

Anti-GAD67 antibody [GAD1/2391] used in Western Blot (WB). GTX17736
Anti-GAD67 antibody [GAD1/2391] used in IHC (Paraffin sections) (IHC-P). GTX17736
Anti-GAD67 antibody [GAD1/2391] used in Protein Array (Protein Array). GTX17736

Cat. No. GTX17736

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GAD1/2391

Isotype

 IgG1

Application

 WB, IHC-P, ELISA, Protein Array

Reactivity

 Human
Package
100 μg ($529)
See all GAD67 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1-2μg/ml
IHC-P 1-2μg/ml for 30 minutes at RT
ELISA Assay dependent
Protein Array Assay dependent

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.

ELISA
For ELISA coating, recommend using BSA-free format (please contact us for PBS only format).
Not tested in other applications.

Calculated MW

67 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human GAD1 (GAD67) protein fragment (around aa 72-135) (exact sequence is proprietary)

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CPSQ1 , GAD , GAD1 , SCP , glutamate decarboxylase 1 , GAD67

Background

This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantigen and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Deficiency in this enzyme has been shown to lead to pyridoxine dependency with seizures. Alternative splicing of this gene results in two products, the predominant 67-kD form and a less-frequent 25-kD form. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-GAD67 antibody [GAD1/2391] used in Western Blot (WB). GTX17736

GTX17736 WB Image

WB analysis of human brain tissue lysate using GTX17736 GAD67 antibody [GAD1/2391].

Anti-GAD67 antibody [GAD1/2391] used in IHC (Paraffin sections) (IHC-P). GTX17736

GTX17736 IHC-P Image

IHC-P analysis of human brain tissue using GTX17736 GAD67 antibody [GAD1/2391].

Anti-GAD67 antibody [GAD1/2391] used in Protein Array (Protein Array). GTX17736

GTX17736 Protein Array Image

Analysis of Protein Array containing more than 19,000 full-length human proteins using GAD1 (GAD67) Mouse Monoclonal Antibody (GAD1/2391). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target if the Monoclonal Antibody has an S-score of at least 2.5. For example, if a Monoclonal Antibody binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Monoclonal Antibody to protein X is equal to 29.

REFERENCE

There are currently no references for GAD67 antibody [GAD1/2391] (GTX17736). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for GAD67 antibody [GAD1/2391] (GTX17736). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 529