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GAD67 antibody [GT1982]

Overexpression
Anti-GAD67 antibody [GT1982] used in IHC (Paraffin sections) (IHC-P). GTX633816
Anti-GAD67 antibody [GT1982] used in Western Blot (WB). GTX633816
Anti-GAD67 antibody [GT1982] used in Western Blot (WB). GTX633816
Anti-GAD67 antibody [GT1982] used in Western Blot (WB). GTX633816
Anti-GAD67 antibody [GT1982] used in Western Blot (WB). GTX633816

Cat. No. GTX633816

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT1982

Isotype

 IgG1

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all GAD67 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

67 kDa. ( Note )

Positive Control

mouse brain , rat brain

Product Note

This antibody is specific for GAD67 protein, and it does not cross-react with GAD65 protein.

Predict Reactivity

Bovine, Cat, Dog, Chicken, Pig, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.24 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human GAD67. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888379

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glutamate decarboxylase 1 , CPSQ1 , GAD , SCP

Background

This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantigen and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Deficiency in this enzyme has been shown to lead to pyridoxine dependency with seizures. Alternative splicing of this gene results in two products, the predominant 67-kD form and a less-frequent 25-kD form. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-GAD67 antibody [GT1982] used in IHC (Paraffin sections) (IHC-P). GTX633816

GTX633816 IHC-P Image

GAD67 antibody [GT1982] detects GAD67 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse eye.
Green: GAD67 stained by GAD67 antibody [GT1982] (GTX633816) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, a cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody (GTX130245) diluted at 1:300.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GAD67 antibody [GT1982] used in Western Blot (WB). GTX633816

GTX633816 WB Image

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with GAD67 antibody[GT1982] (GTX633816) diluted at 1:1000.

Anti-GAD67 antibody [GT1982] used in Western Blot (WB). GTX633816

GTX633816 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GAD67 antibody [GT1982] (GTX633816) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-GAD67 antibody [GT1982] used in Western Blot (WB). GTX633816

GTX633816 WB Image

Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with GAD67 antibody [GT1982] (GTX633816) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-GAD67 antibody [GT1982] used in Western Blot (WB). GTX633816

GTX633816 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GAD67 antibody [GT1982] (GTX633816) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations