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GAD67 antibody [HL1095]

Anti-GAD67 antibody [HL1095] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636279
Anti-GAD67 antibody [HL1095] used in Western Blot (WB). GTX636279
Anti-GAD67 antibody [HL1095] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636279
Anti-GAD67 antibody [HL1095] used in Western Blot (WB). GTX636279
Anti-GAD67 antibody [HL1095] used in Western Blot (WB). GTX636279

Cat No. GTX636279

Host

Rabbit

Clonality

Monoclonal

Clone Name

HL1095

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human, Mouse, Rat
Package
100 μl ($379),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

67 kDa. ( Note )

Product Note

This antibody is specific for GAD67 protein, and it does not cross-react with GAD65 protein.

Predict Reactivity

Bovine, Cat, Dog, Chicken, Pig, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human GAD67. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2909958

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

glutamate decarboxylase 1 , CPSQ1 , GAD , SCP

Background

This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantigen and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Deficiency in this enzyme has been shown to lead to pyridoxine dependency with seizures. Alternative splicing of this gene results in two products, the predominant 67-kD form and a less-frequent 25-kD form. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-GAD67 antibody [HL1095] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636279

GTX636279 ICC/IF Image

GAD67 antibody [HL1095] detects GAD67 protein at cytoplasm by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GAD67 stained by GAD67 antibody [HL1095] (GTX636279) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-GAD67 antibody [HL1095] used in Western Blot (WB). GTX636279

GTX636279 WB Image

Human tissue extract (10 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with GAD67 antibody [HL1095] (GTX636279) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GAD67 antibody [HL1095] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636279

GTX636279 ICC/IF Image

GAD67 antibody [HL1095] detects GAD67 protein by immunofluorescent analysis.
Sample: DIV9 rat cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GAD67 stained by GAD67 antibody [HL1095] (GTX636279) diluted at 1:500.
Red: Tau, a Axon marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.

Anti-GAD67 antibody [HL1095] used in Western Blot (WB). GTX636279

GTX636279 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GAD67 antibody [HL1095] (GTX636279) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GAD67 antibody [HL1095] used in Western Blot (WB). GTX636279

GTX636279 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GAD67 antibody [HL1095] (GTX636279) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 379
$ 169
Custom Conjugations