GeneTex
United States (US)

GAD67 antibody, Internal

GTX88492_944_IHC-P_18122102_462.jpg
GTX88492_2278_WB_18122102_256.jpg

Cat No. GTX88492

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.5-1.5μg/ml
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

67 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88492-PEP).

Specificity/Sensitivity

This antibody is expected to recognize isoform GAD67. There is no cross-reactivity expected with GAD2.

Predict Reactivity

Mouse, Rat, Bovine, Dog, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-PDSPQRREKLHK, from the internal region of the protein sequence according to NP_000808.2.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Glutamate Decarboxylase 1,Cpsq1,Gad,Scp,Gad1

Background

This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantigen and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Deficiency in this enzyme has been shown to lead to pyridoxine dependency with seizures. Alternative splicing of this gene results in two products, the predominant 67-kD form and a less-frequent 25-kD form. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
GTX88492_944_IHC-P_18122102_462.jpg

GTX88492 IHC-P Image

IHC-P analysis of human cerebellum using GTX88492 GAD67 antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 4µg/ml

GTX88492_2278_WB_18122102_256.jpg

GTX88492 WB Image

WB analysis of human brain (cerebral cortex) lysate using GTX88492 GAD67 antibody, Internal.
Dilution : 0.5µg/ml
Loading : 35µg protein in RIPA buffer

Package List Price ($)
$ 329