GeneTex

  • Country / Location Selection

United States (US)

GADD153 antibody [9C8]

Orthogonal
Anti-GADD153 antibody [9C8] used in Western Blot (WB). GTX11419
Anti-GADD153 antibody [9C8] used in Western Blot (WB). GTX11419
Anti-GADD153 antibody [9C8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX11419

Cat. No. GTX11419

Reference 4

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 9C8

Isotype

 IgG2b

Application

 WB, ICC/IF, IHC-P, IHC-Fr, IP

Reactivity

 Human, Mouse, Rat, Rabbit
Package
50 μg ($399)
See all GADD153 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 μg/ml
ICC/IF Assay dependent
IHC-P Assay dependent
IHC-Fr 1:100
IP Assay dependent
Not tested in other applications.

Calculated MW

19 kDa. ( Note )

Product Note

Clone 9C8 has been shown to recognize an epitope in the N-terminal region of CHOP

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

A bacterially expressed, mouse CHOP fusion protein.

Purification

Protein A purified

Conjugation

Unconjugated

RRID

AB_368710

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

DNA damage inducible transcript 3 , AltDDIT3 , C/EBPzeta , CEBPZ , CHOP , CHOP-10 , CHOP10 , GADD153

Cellular Localization

Cytoplasm,Nucleus

Background

This gene encodes a member of the CCAAT/enhancer-binding protein (C/EBP) family of transcription factors. The protein functions as a dominant-negative inhibitor by forming heterodimers with other C/EBP members, such as C/EBP and LAP (liver activator protein), and preventing their DNA binding activity. The protein is implicated in adipogenesis and erythropoiesis, is activated by endoplasmic reticulum stress, and promotes apoptosis. Fusion of this gene and FUS on chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins in myxoid liposarcomas or Ewing sarcoma. Multiple alternatively spliced transcript variants encoding two isoforms with different length have been identified. [provided by RefSeq, Aug 2010]

Database

Research Area

DATA IMAGES

Anti-GADD153 antibody [9C8] used in Western Blot (WB). GTX11419

GTX11419 WB Image

Orthogonal

WB analysis of 30 ug of whole cell lysates from undifferentiated 3T3-L1 fibroblasts (Fib) and 3T3-L1 fibroblasts differentiated into adipocytes (Day 2 and Day 9 post-differentiation) using GTX11419 GADD153 antibody [9C8].
3T3-L1 fibroblasts were cultured in differentiation medium for 3 days to generate adipocytes. Differentiated adipocytes were maintained in maturation media containing either 5mM glucose (normal glucose) or 30mM glucose (high glucose).

Dilution : 1:1000

Anti-GADD153 antibody [9C8] used in Western Blot (WB). GTX11419

GTX11419 WB Image

Orthogonal

WB analysis of HeLa cells were left untreated (-) or treated with tunicamycin (5 μg/ml) for 11 hours (+) using GTX11419 GADD153 antibody [9C8].
Dilution : 1:2000

Anti-GADD153 antibody [9C8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX11419

GTX11419 ICC/IF Image

Orthogonal

ICC/IF analysis of A549 cells treated with media only (non-treated) or with 1.5 μM tunicamycin for 6 hours using GTX11419 GADD153 antibody [9C8].

REFERENCE

Application Reference

Monceaux K et al. Int J Mol Sci 2022; 23 (12) Ferulic Acid, Pterostilbene, and Tyrosol Protect the Heart from ER-Stress-Induced Injury by Activating SIRT1-Dependent Deacetylation of eIF2α.
Guan P et al. Life Sci 2019; 225 : 46-54 Hydrogen protects against chronic intermittent hypoxia induced renal dysfunction by promoting autophagy and alleviating apoptosis.
Application : WB
Reactivity : Rat
Su CC et al. Anticancer Res 2014; 34 (12) : 7097-110 Tanshinone IIA inhibits gastric carcinoma AGS cells through increasing p-p38, p-JNK and p53 but reducing p-ERK, CDC2 and cyclin B1 expression.

Clone Reference

Nozomi Takahashi et al. Sci Rep . 2017; 7 (1):10824 Activation of Endoplasmic Reticulum Stress in Granulosa Cells from Patients with Polycystic Ovary Syndrome Contributes to Ovarian Fibrosis
Application : IHC-P
Reactivity : Human, Mouse
Submit a Reference

REVIEW

There are currently no reviews for GADD153 antibody [9C8] (GTX11419). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399