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GADD153 antibody [HL2262]

Recombinant antibody Orthogonal
Anti-GADD153 antibody [HL2262] used in Western Blot (WB). GTX638314
Anti-GADD153 antibody [HL2262] used in Western Blot (WB). GTX638314
Anti-GADD153 antibody [HL2262] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638314
Anti-GADD153 antibody [HL2262] used in IHC (Paraffin sections) (IHC-P). GTX638314
Anti-GADD153 antibody [HL2262] used in IHC (Paraffin sections) (IHC-P). GTX638314

Cat. No. GTX638314

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2262

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all GADD153 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

19 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human GADD153 recombinant protein.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

DNA damage inducible transcript 3 , AltDDIT3 , C/EBPzeta , CEBPZ , CHOP , CHOP-10 , CHOP10 , GADD153

Cellular Localization

Cytoplasm,Nucleus

Background

This gene encodes a member of the CCAAT/enhancer-binding protein (C/EBP) family of transcription factors. The protein functions as a dominant-negative inhibitor by forming heterodimers with other C/EBP members, such as C/EBP and LAP (liver activator protein), and preventing their DNA binding activity. The protein is implicated in adipogenesis and erythropoiesis, is activated by endoplasmic reticulum stress, and promotes apoptosis. Fusion of this gene and FUS on chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins in myxoid liposarcomas or Ewing sarcoma. Multiple alternatively spliced transcript variants encoding two isoforms with different length have been identified. [provided by RefSeq, Aug 2010]

Database

Research Area

DATA IMAGES

Anti-GADD153 antibody [HL2262] used in Western Blot (WB). GTX638314

GTX638314 WB Image

Orthogonal

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with GADD153 antibody [HL2262] (GTX638314) diluted at 1:4000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GADD153 antibody [HL2262] used in Western Blot (WB). GTX638314

GTX638314 WB Image

Orthogonal

Untreated (–) and treated (+) HepG2 whole cell extract (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with GADD153 antibody [HL2262] (GTX638314) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GADD153 antibody [HL2262] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638314

GTX638314 ICC/IF Image

Orthogonal

GADD153 antibody [HL2262] detects GADD153 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GADD153 stained by GADD153 antibody [HL2262] (GTX638314) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-GADD153 antibody [HL2262] used in IHC (Paraffin sections) (IHC-P). GTX638314

GTX638314 IHC-P Image

GADD153 antibody [HL2262] detects GADD153 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human colon cancer.
GADD153 stained by GADD153 antibody [HL2262] (GTX638314) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GADD153 antibody [HL2262] used in IHC (Paraffin sections) (IHC-P). GTX638314

GTX638314 IHC-P Image

GADD153 antibody [HL2262] detects GADD153 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
GADD153 stained by GADD153 antibody [HL2262] (GTX638314) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations