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United States (US)

GADD153 antibody

Cat. No. GTX10444

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399)
See all GADD153 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:200-1:400
ICC/IF 5-10 μg/ml
Not tested in other applications.

Calculated MW

19 kDa. ( Note )

Positive Control

HeLa

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA

Preservative

15mM Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

peptide corresponding to human GADD153/CHOP10 sequence (amino acids 153-169) with N-terminal lysine added, conjugated to keyhole limpet hemocyanin (KLH). The corresponding sequence in mouse and rat differs by 2 amino acids.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

DNA damage inducible transcript 3 , AltDDIT3 , C/EBPzeta , CEBPZ , CHOP , CHOP-10 , CHOP10 , GADD153

Cellular Localization

Cytoplasm,Nucleus

Background

DDIT3 is a basic region leucine zipper transcription factor, devoid of DNA binding site. It is capable of heterodimerizing with members of the CCAAT/ enhancer binding protein (C/EBP) family of transcription factors. It is both an activating and a repressing factor that is markedly induced in response to many signals such as growth arrest, DNA damage, calcium ionophore, nutrient deprivation, hypoxia, hyperoxia, hyperosmosis, and endoplasmic reticulum stress.

Database

Research Area

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399