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GADD153 antibody

Orthogonal
Anti-GADD153 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109226
Anti-GADD153 antibody used in Western Blot (WB). GTX109226

Cat. No. GTX109226

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all GADD153 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

19 kDa. ( Note )

Predict Reactivity

Mouse, Rat, Bovine, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human GADD153 Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11172201

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

DNA damage inducible transcript 3 , AltDDIT3 , C/EBPzeta , CEBPZ , CHOP , CHOP-10 , CHOP10 , GADD153

Cellular Localization

Nucleus

Background

This gene encodes a member of the CCAAT/enhancer-binding protein (C/EBP) family of transcription factors. The protein functions as a dominant-negative inhibitor by forming heterodimers with other C/EBP members, such as C/EBP and LAP (liver activator protein), and preventing their DNA binding activity. The protein is implicated in adipogenesis and erythropoiesis, is activated by endoplasmic reticulum stress, and promotes apoptosis. Fusion of this gene and FUS on chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins in myxoid liposarcomas or Ewing sarcoma. Multiple alternatively spliced transcript variants encoding two isoforms with different length have been identified. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-GADD153 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109226

GTX109226 ICC/IF Image

GADD153 antibody detects GADD153 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: A549 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GADD153 protein stained by GADD153 antibody (GTX109226) diluted at 1:200.
Red: phalloidin, a cytoskeleton marker, diluted at 1:50.
Scale bar = 10 μm.

Anti-GADD153 antibody used in Western Blot (WB). GTX109226

GTX109226 WB Image

Orthogonal

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with GADD153 antibody (GTX109226) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169