Untreated (–) and treated (+) NIH-3T3 whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with GADD153 antibody (GTX109226) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with GADD153 antibody (GTX109226) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GADD153 antibody detects GADD153 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: A549 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GADD153 protein stained by GADD153 antibody (GTX109226) diluted at 1:200.
Red: phalloidin, a cytoskeleton marker, diluted at 1:50.
Scale bar = 10 μm.