GeneTex
United States (US)

GADD153 antibody

Anti-GADD153 antibody used in Western Blot (WB). GTX32616

Cat No. GTX32616

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
Not tested in other applications.

Calculated MW

19 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 100 to the C-terminus of human DDIT3 (NP_004074.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Dna Damage Inducible Transcript 3,C/Ebpzeta,Cebpz,Chop,Chop-10,Chop10,Gadd153,Ddit3

Background

This gene encodes a member of the CCAAT/enhancer-binding protein (C/EBP) family of transcription factors. The protein functions as a dominant-negative inhibitor by forming heterodimers with other C/EBP members, such as C/EBP and LAP (liver activator protein), and preventing their DNA binding activity. The protein is implicated in adipogenesis and erythropoiesis, is activated by endoplasmic reticulum stress, and promotes apoptosis. Fusion of this gene and FUS on chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins in myxoid liposarcomas or Ewing sarcoma. Multiple alternatively spliced transcript variants encoding two isoforms with different length have been identified. [provided by RefSeq, Aug 2010]

Database

Research Area

DATA IMAGES
Anti-GADD153 antibody used in Western Blot (WB). GTX32616

GTX32616 WB Image

WB analysis of various samples using GTX32616 GADD153 antibody.The signal was developed with ECL plus-Enhanced.
Dilution : 1:1000
Loading : 25μg per lane

REFERENCE
Package List Price ($)
$ 329