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GADD153 antibody

Orthogonal
Anti-GADD153 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32616
Anti-GADD153 antibody used in Western Blot (WB). GTX32616
Anti-GADD153 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32616
Anti-GADD153 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32616
Anti-GADD153 antibody used in IHC (Paraffin sections) (IHC-P). GTX32616
Anti-GADD153 antibody used in Western Blot (WB). GTX32616

Cat. No. GTX32616

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all GADD153 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

19 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 100 to the C-terminus of human DDIT3 (NP_004074.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

RRID

AB_2887669

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

DNA damage inducible transcript 3 , AltDDIT3 , C/EBPzeta , CEBPZ , CHOP , CHOP-10 , CHOP10 , GADD153

Cellular Localization

Cytoplasm,Nucleus

Background

This gene encodes a member of the CCAAT/enhancer-binding protein (C/EBP) family of transcription factors. The protein functions as a dominant-negative inhibitor by forming heterodimers with other C/EBP members, such as C/EBP and LAP (liver activator protein), and preventing their DNA binding activity. The protein is implicated in adipogenesis and erythropoiesis, is activated by endoplasmic reticulum stress, and promotes apoptosis. Fusion of this gene and FUS on chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins in myxoid liposarcomas or Ewing sarcoma. Multiple alternatively spliced transcript variants encoding two isoforms with different length have been identified. [provided by RefSeq, Aug 2010]

Database

Research Area

DATA IMAGES

Anti-GADD153 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32616

GTX32616 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX32616 GADD153 antibody.
Blue : DAPI
Dilution : 1:100

Anti-GADD153 antibody used in Western Blot (WB). GTX32616

GTX32616 WB Image

WB analysis of various sample lysates using GTX32616 GADD153 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-GADD153 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32616

GTX32616 ICC/IF Image

ICC/IF analysis of L929 cells using GTX32616 GADD153 antibody.
Blue : DAPI
Dilution : 1:100

Anti-GADD153 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32616

GTX32616 ICC/IF Image

ICC/IF analysis of C6 cells using GTX32616 GADD153 antibody.
Blue : DAPI
Dilution : 1:100

Anti-GADD153 antibody used in IHC (Paraffin sections) (IHC-P). GTX32616

GTX32616 IHC-P Image

IHC-P analysis of rat testis tissue using GTX32616 GADD153 antibody.
Dilution : 1:100

Anti-GADD153 antibody used in Western Blot (WB). GTX32616

GTX32616 WB Image

Orthogonal

The data was published in the journal Oxid Med Cell Longev in 2019. PMID: 30984338

REFERENCE

Application Reference

Wang P et al. Redox Biol 2022; 57 : 102475 Mitochondrial ferritin alleviates apoptosis by enhancing mitochondrial bioenergetics and stimulating glucose metabolism in cerebral ischemia reperfusion.
Zhao YS et al. Oxid Med Cell Longev 2019; 2019 : 7415212 Hydrogen and Oxygen Mixture to Improve Cardiac Dysfunction and Myocardial Pathological Changes Induced by Intermittent Hypoxia in Rats.
Application : WB
Reactivity : Rat
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399