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GADD34 antibody, C-term

Anti-GADD34 antibody, C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX74479
Anti-GADD34 antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX74479
Anti-GADD34 antibody, C-term used in Western Blot (WB). GTX74479
Anti-GADD34 antibody, C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX74479
Anti-GADD34 antibody, C-term used in Flow cytometry (FACS). GTX74479

Cat. No. GTX74479

Reference 1

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, FACS

Reactivity

 Human
Package
100 μg ($399)
See all GADD34 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.3-1μg/ml
ICC/IF 10μg/ml
IHC-P 2-4μg/ml
FACS Assay dependent

Note :

ICC/IF
Strong expression of the protein seen in the nucleus of HeLa and HepG2 cells.

IHC-P
Human Liver shows cytoplasmic staining consistent with ER in the hepatocytes.
Not tested in other applications.

Calculated MW

73 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX74479-PEP).

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-AAALDLSGRRG, from the C Terminus of the protein sequence according to NP_055145.3.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

RRID

AB_377716

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

protein phosphatase 1 regulatory subunit 15A , GADD34

Background

This gene is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The induction of this gene by ionizing radiation occurs in certain cell lines regardless of p53 status, and its protein response is correlated with apoptosis following ionizing radiation. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-GADD34 antibody, C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX74479

GTX74479 ICC/IF Image

ICC/IF analysis of PFA fixed HepG2 cells using GTX74479 GADD34 antibody, C-term.
Green : Primary antibody
Blue : DAPI
Negative control : Unimmunized goat IgG
Permeabilization : 0.15% Triton
Dilution : 10μg/ml

Anti-GADD34 antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX74479

GTX74479 IHC-P Image

IHC-P analysis of human liver using GTX74479 GADD34 antibody, C-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 2μg/ml

Anti-GADD34 antibody, C-term used in Western Blot (WB). GTX74479

GTX74479 WB Image

WB analysis of HepG2 (A) and negative control KLY (B) lysate using GTX74479 GADD34 antibody, C-term.
Dilution : 0.3μg/ml
Loading : 35μg protein in RIPA buffer

Anti-GADD34 antibody, C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX74479

GTX74479 ICC/IF Image

ICC/IF analysis of PFA fixed HeLa cells using GTX74479 GADD34 antibody, C-term.
Green : Primary antibody
Blue : DAPI
Negative control : Unimmunized goat IgG
Permeabilization : 0.15% Triton
Dilution : 10μg/ml

Anti-GADD34 antibody, C-term used in Flow cytometry (FACS). GTX74479

GTX74479 FACS Image

FACS analysis of PFA fixed HepG2 cells using GTX74479 GADD34 antibody, C-term.
Blue : Primary antibody
Black : Isotype control
Permeabilization : 0.5% Triton
Dilution : 10μg/ml

REFERENCE

Application Reference

Song P et al. J Biol Chem 2019; (Epub) The regulatory protein GADD34 inhibits TRAIL-induced apoptosis via TRAF6/ERK-dependent stabilization of myeloid cell leukemia 1 in liver cancer cells.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399