APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500 |
1:50 |
Assay dependent |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS, 50% Glycerol
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Rat
Immunogen
Recombinant rat GAK (kinase domain, 1-430)
Purification
Purified IgG
From culture supernant
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
cyclin G associated kinase , DNAJ26 , DNAJC26 , GAK
Cellular Localization
Cytoplasm,Perinuclear region,Golgi apparatus,trans-Golgi network,Cell junction,Focal adhesion
Background
In all eukaryotes, the cell cycle is governed by cyclin-dependent protein kinases (CDKs), whose activities are regulated by cyclins and CDK inhibitors in a diverse array of mechanisms that involve the control of phosphorylation and dephosphorylation of Ser, Thr or Tyr residues. Cyclins are molecules that possess a consensus domain called the 'cyclin box.' In mammalian cells, 9 cyclin species have been identified, and they are referred to as cyclins A through I. Cyclin G is a direct transcriptional target of the p53 tumor suppressor gene product and thus functions downstream of p53. GAK is an association partner of cyclin G and CDK5. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2015]
Database
Research Area
DATA IMAGES
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GTX00874 ICC/IF Image
ICC/IF analysis of TIG-1 cells using GTX00874 GAK antibody [9-10]. Dilution : 1:50
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GTX00874 WB Image
WB analysis of TIG-1, HeLa S3 and PC-3 whole cell lysates using GTX00874 GAK antibody [9-10]. Dilution : 1:500
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GTX00874 IHC-P Image
IHC-P analysis of human prostate cancer tissue section using GTX00874 GAK antibody [9-10]. It shows nuclear accumulation of GAK in cancer cells. Dilution : 1:50
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REFERENCE
There are currently no references for GAK antibody [9-10] (GTX00874). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for GAK antibody [9-10] (GTX00874). Be the first to share your experience with this product.