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GALE antibody [AT6G10]

Anti-GALE antibody [AT6G10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57657
Anti-GALE antibody [AT6G10] used in Western Blot (WB). GTX57657

Cat. No. GTX57657

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 AT6G10

Isotype

 IgG1

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399)
See all GALE products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

38 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 10% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The clone AT6G10 is derived from hybridization of mouse F2 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human GALE protein.

Purification

Protein A Purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

UDP-galactose-4-epimerase , GALE , SDR1E1 , UDPgalactose4epimerase

Background

This gene encodes UDP-galactose-4-epimerase which catalyzes two distinct but analogous reactions: the epimerization of UDP-glucose to UDP-galactose, and the epimerization of UDP-N-acetylglucosamine to UDP-N-acetylgalactosamine. The bifunctional nature of the enzyme has the important metabolic consequence that mutant cells (or individuals) are dependent not only on exogenous galactose, but also on exogenous N-acetylgalactosamine as a necessary precursor for the synthesis of glycoproteins and glycolipids. Mutations in this gene result in epimerase-deficiency galactosemia, also referred to as galactosemia type 3, a disease characterized by liver damage, early-onset cataracts, deafness and cognitive disability, with symptoms ranging from mild ('peripheral' form) to severe ('generalized' form). Multiple alternatively spliced transcripts encoding the same protein have been identified. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-GALE antibody [AT6G10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57657

GTX57657 ICC/IF Image

ICC/IF analysis of Hep3B cells using GTX57657 GALE antibody.
Blue: DAPI
Green: Primary antibody
Dilution: 1:100

Anti-GALE antibody [AT6G10] used in Western Blot (WB). GTX57657

GTX57657 WB Image

WB analysis of various samples using GTX57657 GALE antibody.
Lane 1 : MCF-7 whole cell lysate
Lane 2 : Jurkat whole cell lysate
Lane 3 : A431 whole cell lysate
Lane 4 : A549 whole cell lysate
Lane 5 : HeLa whole cell lysate
Lane 6 : HepG2 whole cell lysate
Loading : 40 μg
Dilution : 1:1000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399