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GALK2 antibody

Anti-GALK2 antibody used in Western Blot (WB). GTX111750
Anti-GALK2 antibody used in Western Blot (WB). GTX111750
Anti-GALK2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111750
Anti-GALK2 antibody used in IHC (Paraffin sections) (IHC-P). GTX111750

Cat. No. GTX111750

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all GALK2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Positive Control

mouse kidney , HepG2

Predict Reactivity

Rat, Bovine, Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human GALK2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950330

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

galactokinase 2 , GK2

Background

This gene encodes a highly efficient N-acetylgalactosamine (GalNAc) kinase, which has galactokinase activity when galactose is present at high concentrations. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-GALK2 antibody used in Western Blot (WB). GTX111750

GTX111750 WB Image

GALK2 antibody detects GALK2 protein by Western blot analysis.
A. 50 μg mouse kidney lysate/extract
10 % SDS-PAGE
GALK2 antibody (GTX111750) dilution: 1:1000

Anti-GALK2 antibody used in Western Blot (WB). GTX111750

GTX111750 WB Image

Sample (30 ug of whole cell lysate)
A: Hep G2 (GTX27900)
10% SDS PAGE
GTX111750 diluted at 1:5000

Anti-GALK2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111750

GTX111750 ICC/IF Image

Immunofluorescence analysis of methanol-fixed Hep3B, using GALK2(GTX111750) antibody at 1:500 dilution.

Anti-GALK2 antibody used in IHC (Paraffin sections) (IHC-P). GTX111750

GTX111750 IHC-P Image

Immunohistochemical analysis of paraffin-embedded MCF7 xenograft, using GALK2(GTX111750) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169