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GALNS antibody

Anti-GALNS antibody used in Western Blot (WB). GTX110237
Anti-GALNS antibody used in Western Blot (WB). GTX110237
Anti-GALNS antibody used in Western Blot (WB). GTX110237
Anti-GALNS antibody used in IHC (Paraffin sections) (IHC-P). GTX110237
Anti-GALNS antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110237

Cat. No. GTX110237

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all GALNS products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

58 kDa. ( Note )

Positive Control

H1299 , Neuro2A , C2C12 , PC-12 , Rat2

Predict Reactivity

Zebrafish, Bovine, Dog, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human GALNS. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950332

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

galactosamine (N-acetyl)-6-sulfatase , GALNAC6S , GAS , GalN6S , MPS4A

Cellular Localization

Lysosome

Background

This gene encodes N-acetylgalactosamine-6-sulfatase which is a lysosomal exohydrolase required for the degradation of the glycosaminoglycans, keratan sulfate, and chondroitin 6-sulfate. Sequence alterations including point, missense and nonsense mutations, as well as those that affect splicing, result in a deficiency of this enzyme. Deficiencies of this enzyme lead to Morquio A syndrome, a lysosomal storage disorder. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-GALNS antibody used in Western Blot (WB). GTX110237

GTX110237 WB Image

GALNS antibody detects GALNS protein by western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
B. 30 μg Rat2 whole cell lysate/extract
7.5% SDS-PAGE
GALNS antibody (GTX110237) dilution: 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GALNS antibody used in Western Blot (WB). GTX110237

GTX110237 WB Image

Sample (30 μg of whole cell lysate)
A: H1299
7.5% SDS PAGE
GTX110237 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GALNS antibody used in Western Blot (WB). GTX110237

GTX110237 WB Image

GALNS antibody detects GALNS protein by western blot analysis.
A. 30 μg Neuro2A whole cell lysate/extract
B. 30 μg C2C12 whole cell lysate/extract
7.5% SDS-PAGE
GALNS antibody (GTX110237) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GALNS antibody used in IHC (Paraffin sections) (IHC-P). GTX110237

GTX110237 IHC-P Image

Immunohistochemical analysis of paraffin-embedded NCI-N87 xenograft, using GALNS(GTX110237) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-GALNS antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110237

GTX110237 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using GALNS(GTX110237) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:500.

REFERENCE

Application Reference

Bussi C et al. bioRxiv 2017; (Epub) Alpha-synuclein fibrils induce autophagy in microglial cells as a consequence of lysosomal damage.
Application : ICC/IF
Reactivity : Mouse
Submit a Reference

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169