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GALNS antibody

Anti-GALNS antibody used in IHC (Paraffin sections) (IHC-P). GTX110237
Anti-GALNS antibody used in Western Blot (WB). GTX110237
Anti-GALNS antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110237
Anti-GALNS antibody used in Western Blot (WB). GTX110237
Anti-GALNS antibody used in Western Blot (WB). GTX110237

Cat No. GTX110237

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

58 kDa. ( Note )

Positive Control

H1299 , Neuro2A , C2C12 , PC-12 , Rat2

Predict Reactivity

Zebrafish, Bovine, Dog, Pig, Xenopus laevis, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human GALNS. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950332

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

galactosamine (N-acetyl)-6-sulfatase , GALNAC6S , GAS , GalN6S , MPS4A

Cellular Localization

Lysosome

Background

This gene encodes N-acetylgalactosamine-6-sulfatase which is a lysosomal exohydrolase required for the degradation of the glycosaminoglycans, keratan sulfate, and chondroitin 6-sulfate. Sequence alterations including point, missense and nonsense mutations, as well as those that affect splicing, result in a deficiency of this enzyme. Deficiencies of this enzyme lead to Morquio A syndrome, a lysosomal storage disorder. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-GALNS antibody used in IHC (Paraffin sections) (IHC-P). GTX110237

GTX110237 IHC-P Image

Immunohistochemical analysis of paraffin-embedded NCI-N87 xenograft, using GALNS(GTX110237) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-GALNS antibody used in Western Blot (WB). GTX110237

GTX110237 WB Image

Sample (30 μg of whole cell lysate)
A: H1299
7.5% SDS PAGE
GTX110237 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GALNS antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110237

GTX110237 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using GALNS(GTX110237) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:500.

Anti-GALNS antibody used in Western Blot (WB). GTX110237

GTX110237 WB Image

GALNS antibody detects GALNS protein by western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
B. 30 μg Rat2 whole cell lysate/extract
7.5% SDS-PAGE
GALNS antibody (GTX110237) dilution: 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GALNS antibody used in Western Blot (WB). GTX110237

GTX110237 WB Image

GALNS antibody detects GALNS protein by western blot analysis.
A. 30 μg Neuro2A whole cell lysate/extract
B. 30 μg C2C12 whole cell lysate/extract
7.5% SDS-PAGE
GALNS antibody (GTX110237) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169