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GALNT2 antibody

Orthogonal
Anti-GALNT2 antibody used in Western Blot (WB). GTX104070
Anti-GALNT2 antibody used in IHC (Paraffin sections) (IHC-P). GTX104070
Anti-GALNT2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104070
Anti-GALNT2 antibody used in Western Blot (WB). GTX104070
Anti-GALNT2 antibody used in Western Blot (WB). GTX104070
Anti-GALNT2 antibody used in Western Blot (WB). GTX104070

Cat. No. GTX104070

Reference 1
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all GALNT2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

65 kDa. ( Note )

Positive Control

Mouse white adipose tissue

Predict Reactivity

Zebrafish, Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.77 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human GALNT2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950333

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

polypeptide N-acetylgalactosaminyltransferase 2 , GalNAc-T2

Cellular Localization

Golgi apparatus , Golgi stack membrane; Single-pass type II membrane protein , Secreted

Background

This gene encodes polypeptide N-acetylgalactosaminyltransferase 2, a member of the GalNAc-transferases family. This family transfers an N-acetyl galactosamine to the hydroxyl group of a serine or threonine residue in the first step of O-linked oligosaccharide biosynthesis. Individual GalNAc-transferases have distinct activities and initiation of O-glycosylation in a cell is regulated by a repertoire of GalNAc-transferases. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-GALNT2 antibody used in Western Blot (WB). GTX104070

GTX104070 WB Image

Sample (50 μg of whole cell lysate)
A: Mouse white adipose tissue
7.5% SDS PAGE
GTX104070 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GALNT2 antibody used in IHC (Paraffin sections) (IHC-P). GTX104070

GTX104070 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using GALNT2(GTX104070) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-GALNT2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104070

GTX104070 ICC/IF Image

GALNT2 antibody detects GALNT2 protein at Golgi apparatus by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: GALNT2 stained by GALNT2 antibody (GTX104070) diluted at 1:500.
Red: alpha Tubulin, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10 μm.

Anti-GALNT2 antibody used in Western Blot (WB). GTX104070

GTX104070 WB Image

Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with GALNT2 antibody (GTX104070) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GALNT2 antibody used in Western Blot (WB). GTX104070

GTX104070 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GALNT2 antibody (GTX104070) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GALNT2 antibody used in Western Blot (WB). GTX104070

GTX104070 WB Image

Orthogonal

The data was published in the journal PLoS One in 2016. PMID: 27281159

REFERENCE

Application Reference

Nagai-Okatani C et al. PLoS One 2016; 11 (6) : e0150210 Aberrant Glycosylation in the Left Ventricle and Plasma of Rats with Cardiac Hypertrophy and Heart Failure.
Application : WB
Reactivity : Rat
Submit a Reference

REVIEW

GALNT2 antibody Cat. No. GTX104070

Rating
( Average 4 based on 1 users reviews)
Application
Western Blot(WB)
( Average 4 based on 1 users reviews)
Anti-GALNT2 antibody used in Western Blot (WB). GTX104070
Date : Anonymous submitted on 31-Jul-2020
Score :
Application Tested : WB
Sample Species : Hu
Sample : H4, SH-SY5Y cells
Amount used : 4 μg
Blocking : 0.5 % casein in TBST, 25°C, 1Hr
Primary Antibody : 1:1500, 4°C, 16Hr
Figure Note : Image courtesy of Nobuhisa Iwata from Nagasaki University.
Submit a Review
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169