Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GALNT2 antibody (GTX104070) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

GALNT2 antibody detects GALNT2 protein at Golgi apparatus by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: GALNT2 stained by GALNT2 antibody (GTX104070) diluted at 1:500.
Red: alpha Tubulin, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10 μm.

Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with GALNT2 antibody (GTX104070) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using GALNT2(GTX104070) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Sample (50 μg of whole cell lysate)
A: Mouse white adipose tissue
7.5% SDS PAGE
GTX104070 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

The data was published in the journal PLoS One in 2016. PMID: 27281159