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GALNT3 antibody

Anti-GALNT3 antibody used in IHC (Paraffin sections) (IHC-P). GTX131628
Anti-GALNT3 antibody used in IHC (Paraffin sections) (IHC-P). GTX131628
Anti-GALNT3 antibody used in Western Blot (WB). GTX131628
Anti-GALNT3 antibody used in IHC (Paraffin sections) (IHC-P). GTX131628
Anti-GALNT3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX131628
Anti-GALNT3 antibody used in IHC (Paraffin sections) (IHC-P). GTX131628
Anti-GALNT3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX131628

Cat. No. GTX131628

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all GALNT3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

73 kDa. ( Note )

Predict Reactivity

Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.31 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human GALNT3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886509

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

polypeptide N-acetylgalactosaminyltransferase 3 , GalNAc-T3 , HFTC , HFTC1 , HHS

Background

This gene encodes UDP-GalNAc transferase 3, a member of the GalNAc-transferases family. This family transfers an N-acetyl galactosamine to the hydroxyl group of a serine or threonine residue in the first step of O-linked oligosaccharide biosynthesis. Individual GalNAc-transferases have distinct activities and initiation of O-glycosylation is regulated by a repertoire of GalNAc-transferases. The protein encoded by this gene is highly homologous to other family members, however the enzymes have different substrate specificities. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-GALNT3 antibody used in IHC (Paraffin sections) (IHC-P). GTX131628

GTX131628 IHC-P Image

GALNT3 antibody detects GALNT3 protein at Golgi apparatus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse stomach.
GALNT3 stained by GALNT3 antibody (GTX131628) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GALNT3 antibody used in IHC (Paraffin sections) (IHC-P). GTX131628

GTX131628 IHC-P Image

GALNT3 antibody detects GALNT3 protein at Golgi apparatus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
GALNT3 stained by GALNT3 antibody (GTX131628) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GALNT3 antibody used in Western Blot (WB). GTX131628

GTX131628 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GALNT3 antibody (GTX131628) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GALNT3 antibody used in IHC (Paraffin sections) (IHC-P). GTX131628

GTX131628 IHC-P Image

GALNT3 antibody detects GALNT3 protein at Golgi apparatus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
GALNT3 stained by GALNT3 antibody (GTX131628) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GALNT3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX131628

GTX131628 ICC/IF Image

GALNT3 antibody detects GALNT3 protein at Golgi apparatus by immunofluorescent analysis.
Sample: A431 cells were fixed in ice-cold MeOH for 5 min.
Green: GALNT3 stained by GALNT3 antibody (GTX131628) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar= 10 μm.

Anti-GALNT3 antibody used in IHC (Paraffin sections) (IHC-P). GTX131628

GTX131628 IHC-P Image

GALNT3 antibody detects GALNT3 protein at Golgi apparatus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse stomach.
GALNT3 stained by GALNT3 antibody (GTX131628) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GALNT3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX131628

GTX131628 ICC/IF Image

GALNT3 antibody detects GALNT3 protein at Golgi by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GALNT3 stained by GALNT3 antibody (GTX131628) diluted at 1:3000.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169