APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| Assay dependent |
Not tested in other applications.
Calculated MW
Product Note
This antibody is specific for human GALNT6 protein, and it does not cross react with human GALNT3 protein.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant fragment of human GALNT6.
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
polypeptide N-acetylgalactosaminyltransferase 6 , GALNAC-T6 , GalNAcT6
Cellular Localization
Golgi apparatus membrane
Background
This gene encodes a member of the UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase (GalNAc-T) family of enzymes. GalNAc-Ts initiate mucin-type O-linked glycosylation in the Golgi apparatus by catalyzing the transfer of GalNAc to serine and threonine residues on target proteins. They are characterized by an N-terminal transmembrane domain, a stem region, a lumenal catalytic domain containing a GT1 motif and Gal/GalNAc transferase motif, and a C-terminal ricin/lectin-like domain. GalNAc-Ts have different, but overlapping, substrate specificities and patterns of expression. The encoded protein is capable of glycosylating fibronectin peptide in vitro and is expressed in a fibroblast cell line, indicating that it may be involved in the synthesis of oncofetal fibronectin. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
![Anti-GALNT6 antibody [HL2392] used in IHC (Paraffin sections) (IHC-P). GTX638601](/templates/default/js/lightbox/images/loading.gif "Anti-GALNT6 antibody [HL2392] used in IHC (Paraffin sections) (IHC-P). GTX638601")
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GTX638601 IHC-P Image
GALNT6 antibody [HL2392] detects GALNT6 protein at Golgi apparatus by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissue.GALNT6 stained by GALNT6 antibody [HL2392] (GTX638601) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX638601 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GALNT6 antibody [HL2392] (GTX638601) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX638601 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GALNT6 antibody [HL2392] (GTX638601) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
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GTX638601 WB Image
Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with GALNT6 antibody [HL2392] (GTX638601) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX638601 WB Image
Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GALNT6 antibody [HL2392] (GTX638601) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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REFERENCE
There are currently no references for GALNT6 antibody [HL2392] (GTX638601). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for GALNT6 antibody [HL2392] (GTX638601). Be the first to share your experience with this product.
![Anti-GALNT6 antibody [HL2392] used in IHC (Paraffin sections) (IHC-P). GTX638601](/upload/website/prouct_img/small/GTX638601/GTX638601_T-45047_20230602_IHC-P_multiple_M_23061320_300.webp "Anti-GALNT6 antibody [HL2392] used in IHC (Paraffin sections) (IHC-P). GTX638601")
![Anti-GALNT6 antibody [HL2392] used in Western Blot (WB). GTX638601](/upload/website/prouct_img/small/GTX638601/GTX638601_T-45047_20230602_WB_multiple_B_23060622_199.webp "Anti-GALNT6 antibody [HL2392] used in Western Blot (WB). GTX638601")
![Anti-GALNT6 antibody [HL2392] used in Western Blot (WB). GTX638601](/upload/website/prouct_img/small/GTX638601/GTX638601_45117_20230728_WB_TPM_watermark_23073119_571.webp "Anti-GALNT6 antibody [HL2392] used in Western Blot (WB). GTX638601")
![Anti-GALNT6 antibody [HL2392] used in Western Blot (WB). GTX638601](/upload/website/prouct_img/small/GTX638601/GTX638601_45117_20230818_WB_R_stomach_23082201_677.webp "Anti-GALNT6 antibody [HL2392] used in Western Blot (WB). GTX638601")
![Anti-GALNT6 antibody [HL2392] used in Western Blot (WB). GTX638601](/upload/website/prouct_img/small/GTX638601/GTX638601_T-45047_20230526_WB_M_tissue_23053001_590.webp "Anti-GALNT6 antibody [HL2392] used in Western Blot (WB). GTX638601")
![Anti-GALNT6 antibody [HL2392] used in IHC (Paraffin sections) (IHC-P). GTX638601](/upload/website/prouct_img/normal/GTX638601/GTX638601_T-45047_20230602_IHC-P_multiple_M_23061320_300.webp "Anti-GALNT6 antibody [HL2392] used in IHC (Paraffin sections) (IHC-P). GTX638601")
![Anti-GALNT6 antibody [HL2392] used in Western Blot (WB). GTX638601](/upload/website/prouct_img/normal/GTX638601/GTX638601_T-45047_20230602_WB_multiple_B_23060622_199.webp "Anti-GALNT6 antibody [HL2392] used in Western Blot (WB). GTX638601")
![Anti-GALNT6 antibody [HL2392] used in Western Blot (WB). GTX638601](/upload/website/prouct_img/normal/GTX638601/GTX638601_45117_20230728_WB_TPM_watermark_23073119_571.webp "Anti-GALNT6 antibody [HL2392] used in Western Blot (WB). GTX638601")
![Anti-GALNT6 antibody [HL2392] used in Western Blot (WB). GTX638601](/upload/website/prouct_img/normal/GTX638601/GTX638601_45117_20230818_WB_R_stomach_23082201_677.webp "Anti-GALNT6 antibody [HL2392] used in Western Blot (WB). GTX638601")
![Anti-GALNT6 antibody [HL2392] used in Western Blot (WB). GTX638601](/upload/website/prouct_img/normal/GTX638601/GTX638601_T-45047_20230526_WB_M_tissue_23053001_590.webp "Anti-GALNT6 antibody [HL2392] used in Western Blot (WB). GTX638601")