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GALNT6 antibody [N1C2]

Orthogonal
Anti-GALNT6 antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104602
Anti-GALNT6 antibody [N1C2] used in Western Blot (WB). GTX104602
Anti-GALNT6 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX104602
Anti-GALNT6 antibody [N1C2] used in Western Blot (WB). GTX104602
Anti-GALNT6 antibody [N1C2] used in Western Blot (WB). GTX104602

Cat. No. GTX104602

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC

Reactivity

 Human, Rat
Package
100 μl ($399),
25 μl ($169)
See all GALNT6 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC Assay dependent
Not tested in other applications.

Calculated MW

71 kDa. ( Note )

Positive Control

A431 , PC-12

Predict Reactivity

Mouse, Bovine, Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.17 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human GALNT6. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950334

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

polypeptide N-acetylgalactosaminyltransferase 6 , GALNAC-T6 , GalNAcT6

Cellular Localization

Golgi apparatus membrane; Single-pass type II membrane protein

Background

This gene encodes a member of the UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase (GalNAc-T) family of enzymes. GalNAc-Ts initiate mucin-type O-linked glycosylation in the Golgi apparatus by catalyzing the transfer of GalNAc to serine and threonine residues on target proteins. They are characterized by an N-terminal transmembrane domain, a stem region, a lumenal catalytic domain containing a GT1 motif and Gal/GalNAc transferase motif, and a C-terminal ricin/lectin-like domain. GalNAc-Ts have different, but overlapping, substrate specificities and patterns of expression. The encoded protein is capable of glycosylating fibronectin peptide in vitro and is expressed in a fibroblast cell line, indicating that it may be involved in the synthesis of oncofetal fibronectin. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-GALNT6 antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104602

GTX104602 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A431, using GALNT6(GTX104602) antibody at 1:200 dilution.

Anti-GALNT6 antibody [N1C2] used in Western Blot (WB). GTX104602

GTX104602 WB Image

Sample(30 ug whole cell lysate)
A:A431(GTX27909)
7.5% SDS PAGE
GTX104602 diluted at 1:1000

Anti-GALNT6 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX104602

GTX104602 IHC-P Image

Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using GALNT6(GTX104602) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-GALNT6 antibody [N1C2] used in Western Blot (WB). GTX104602

GTX104602 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with GALNT6 antibody [N1C2] (GTX104602) diluted at 1:1000.

Anti-GALNT6 antibody [N1C2] used in Western Blot (WB). GTX104602

GTX104602 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GALNT6 antibody [N1C2] (GTX104602) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

REFERENCE

Application Reference

Liesche F et al. Mol Med Rep 2016; (Epub) Role of N-acetylgalactosaminyltransferase 6 in early tumorigenesis and formation of metastasis.
Application : ICC/IF
Andergassen U et al. Biomed Res Int 2015; 2015 (Epub) : 792672 Glycosyltransferases as Markers for Early Tumorigenesis.
Application : IHC
Reactivity : Human
Submit a Reference

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169