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GALNT6 antibody [N1C2]

Anti-GALNT6 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX104602
Anti-GALNT6 antibody [N1C2] used in Western Blot (WB). GTX104602
Anti-GALNT6 antibody [N1C2] used in Western Blot (WB). GTX104602
Anti-GALNT6 antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104602

Cat No. GTX104602

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC
Reactivity Human, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC Assay dependent
Not tested in other applications.

Calculated MW

71 kDa. ( Note )

Positive Control

293T , A431 , H1299 , Molt-4 , PC-12

Predict Reactivity

Mouse, Bovine, Chicken, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.79 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human GALNT6. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

polypeptide N-acetylgalactosaminyltransferase 6 , GALNAC-T6 , GalNAcT6

Cellular Localization

Golgi apparatus membrane; Single-pass type II membrane protein

Background

This gene encodes a member of the UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase (GalNAc-T) family of enzymes. GalNAc-Ts initiate mucin-type O-linked glycosylation in the Golgi apparatus by catalyzing the transfer of GalNAc to serine and threonine residues on target proteins. They are characterized by an N-terminal transmembrane domain, a stem region, a lumenal catalytic domain containing a GT1 motif and Gal/GalNAc transferase motif, and a C-terminal ricin/lectin-like domain. GalNAc-Ts have different, but overlapping, substrate specificities and patterns of expression. The encoded protein is capable of glycosylating fibronectin peptide in vitro and is expressed in a fibroblast cell line, indicating that it may be involved in the synthesis of oncofetal fibronectin. [provided by RefSeq]

Database

DATA IMAGES
Anti-GALNT6 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX104602

GTX104602 IHC-P Image

Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using GALNT6(GTX104602) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-GALNT6 antibody [N1C2] used in Western Blot (WB). GTX104602

GTX104602 WB Image

Whole cell extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with GALNT6 antibody [N1C2] (GTX104602) diluted at 1:1000.

Anti-GALNT6 antibody [N1C2] used in Western Blot (WB). GTX104602

GTX104602 WB Image

Sample(30 ug whole cell lysate)
A:A431(GTX27909)
7.5% SDS PAGE
GTX104602 diluted at 1:1000

Anti-GALNT6 antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104602

GTX104602 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A431, using GALNT6(GTX104602) antibody at 1:200 dilution.

REFERENCE
REVIEW
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169