APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:5000-1:20000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji , mouse brain
Predict Reactivity
Zebrafish, Xenopus laevis(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1mg/ml(Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human GALNT7. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
GALNAC-T7 antibody, GalNAcT7 antibody, GALNT7 antibody, pp-GaNTase 7 antibody, N-acetylgalactosaminyltransferase 7 antibody, polypeptide GalNAc transferase 7 antibody, UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 7 antibody, polypeptide N-acetylgalactosaminyltransferase 7 antibody, protein-UDP acetylgalactosaminyltransferase 7 antibody, UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 7 (GalNAc-T7) antibody
Cellular Localization
Golgi apparatus membrane; Single-pass type II membrane protein
Background
This gene encodes GalNAc transferase 7, a member of the GalNAc-transferase family. The enzyme encoded by this gene controls the initiation step of mucin-type O-linked protein glycosylation and transfer of N-acetylgalactosamine to serine and threonine amino acid residues. This enzyme is a type II transmembrane protein and shares common sequence motifs with other family members. Unlike other family members, this enzyme shows exclusive specificity for partially GalNAc-glycosylated acceptor substrates and shows no activity with non-glycosylated peptides. This protein may function as a follow-up enzyme in the initiation step of O-glycosylation. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX106068 ICC/IF Image
GALNT7 antibody [N1C2] detects GALNT7 protein at Golgi apparatus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GALNT7 protein stained by GALNT7 antibody [N1C2] (GTX106068) diluted at 1:500.
Blue: Hoechst 33343 staining.
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GTX106068 WB Image
Sample (30 µg of whole cell lysate)
A: A431 (GTX27909)
7.5% SDS PAGE
GTX106068 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX106068 IHC-P Image
Immunohistochemical analysis of paraffin-embedded human endometrial cancer, using GALNT7(GTX106068) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX106068 WB Image
Sample (50 µg of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX106068 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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REFERENCE
