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GALNT7 antibody [N1C2]

Anti-GALNT7 antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106068
Anti-GALNT7 antibody [N1C2] used in Western Blot (WB). GTX106068
Anti-GALNT7 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX106068
Anti-GALNT7 antibody [N1C2] used in Western Blot (WB). GTX106068

Cat No. GTX106068

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

75 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji , mouse brain

Predict Reactivity

Zebrafish, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7), 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human GALNT7. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950335

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

polypeptide N-acetylgalactosaminyltransferase 7 , GALNAC-T7 , GalNAcT7

Cellular Localization

Golgi apparatus membrane; Single-pass type II membrane protein

Background

This gene encodes GalNAc transferase 7, a member of the GalNAc-transferase family. The enzyme encoded by this gene controls the initiation step of mucin-type O-linked protein glycosylation and transfer of N-acetylgalactosamine to serine and threonine amino acid residues. This enzyme is a type II transmembrane protein and shares common sequence motifs with other family members. Unlike other family members, this enzyme shows exclusive specificity for partially GalNAc-glycosylated acceptor substrates and shows no activity with non-glycosylated peptides. This protein may function as a follow-up enzyme in the initiation step of O-glycosylation. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-GALNT7 antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106068

GTX106068 ICC/IF Image

GALNT7 antibody [N1C2] detects GALNT7 protein at Golgi apparatus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GALNT7 protein stained by GALNT7 antibody [N1C2] (GTX106068) diluted at 1:500.
Blue: Hoechst 33343 staining.

Anti-GALNT7 antibody [N1C2] used in Western Blot (WB). GTX106068

GTX106068 WB Image

Sample (30 μg of whole cell lysate)
A: A431 (GTX27909)
7.5% SDS PAGE
GTX106068 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GALNT7 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX106068

GTX106068 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human endometrial cancer, using GALNT7(GTX106068) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-GALNT7 antibody [N1C2] used in Western Blot (WB). GTX106068

GTX106068 WB Image

Sample (50 μg of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX106068 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

GALNT7 antibody [N1C2] Cat No. GTX106068

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Rat
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2016. PMID: 27281159
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169