GeneTex
United States (US)

GALNT7 antibody [N1C2]

GTX106068_39974_IFA_19011506_382.jpg
GTX106068_WB_19011506_864.jpg
GTX106068_39974_IHC_19011506_679.jpg
GTX106068_39974_WB_M_brain_19011506_377.jpg

Cat No. GTX106068

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

75 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji , mouse brain

Predict Reactivity

Zebrafish, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human GALNT7. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

GALNAC-T7 antibody, GalNAcT7 antibody, GALNT7 antibody, pp-GaNTase 7 antibody, N-acetylgalactosaminyltransferase 7 antibody, polypeptide GalNAc transferase 7 antibody, UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 7 antibody, polypeptide N-acetylgalactosaminyltransferase 7 antibody, protein-UDP acetylgalactosaminyltransferase 7 antibody, UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 7 (GalNAc-T7) antibody

Cellular Localization

Golgi apparatus membrane; Single-pass type II membrane protein

Background

This gene encodes GalNAc transferase 7, a member of the GalNAc-transferase family. The enzyme encoded by this gene controls the initiation step of mucin-type O-linked protein glycosylation and transfer of N-acetylgalactosamine to serine and threonine amino acid residues. This enzyme is a type II transmembrane protein and shares common sequence motifs with other family members. Unlike other family members, this enzyme shows exclusive specificity for partially GalNAc-glycosylated acceptor substrates and shows no activity with non-glycosylated peptides. This protein may function as a follow-up enzyme in the initiation step of O-glycosylation. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX106068_39974_IFA_19011506_382.jpg

GTX106068 ICC/IF Image

GALNT7 antibody [N1C2] detects GALNT7 protein at Golgi apparatus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GALNT7 protein stained by GALNT7 antibody [N1C2] (GTX106068) diluted at 1:500.
Blue: Hoechst 33343 staining.

GTX106068_WB_19011506_864.jpg

GTX106068 WB Image

Sample (30 µg of whole cell lysate)
A: A431 (GTX27909)
7.5% SDS PAGE
GTX106068 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX106068_39974_IHC_19011506_679.jpg

GTX106068 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human endometrial cancer, using GALNT7(GTX106068) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX106068_39974_WB_M_brain_19011506_377.jpg

GTX106068 WB Image

Sample (50 µg of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX106068 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169