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GALR2 antibody [C1C2], Internal

Anti-GALR2 antibody [C1C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX100320
Anti-GALR2 antibody [C1C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX100320
Anti-GALR2 antibody [C1C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX100320
Anti-GALR2 antibody [C1C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX100320
Anti-GALR2 antibody [C1C2], Internal used in Western Blot (WB). GTX100320
Anti-GALR2 antibody [C1C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100320

Cat No. GTX100320

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human GALR2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

galanin receptor 2 , GAL2-R , GALNR2 , GALR-2

Cellular Localization

Cell membrane; Multi-pass membrane protein

Background

Galanin is an important neuromodulator present in the brain, gastrointestinal system, and hypothalamopituitary axis. It is a 30-amino acid non-C-terminally amidated peptide that potently stimulates growth hormone secretion, inhibits cardiac vagal slowing of heart rate, abolishes sinus arrhythmia, and inhibits postprandial gastrointestinal motility. The actions of galanin are mediated through interaction with specific membrane receptors that are members of the 7-transmembrane family of G protein-coupled receptors. GALR2 interacts with the N-terminal residues of the galanin peptide. The primary signaling mechanism for GALR2 is through the phospholipase C/protein kinase C pathway (via Gq), in contrast to GALR1, which communicates its intracellular signal by inhibition of adenylyl cyclase through Gi. However, it has been demonstrated that GALR2 couples efficiently to both the Gq and Gi proteins to simultaneously activate 2 independent signal transduction pathways. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-GALR2 antibody [C1C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX100320

GTX100320 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human skin, using Galanin Receptor 2(GTX100320) antibody(10 μg/ml).
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-GALR2 antibody [C1C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX100320

GTX100320 IHC-P Image

GALR2 antibody [C1C2], Internal detects GALR2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
GALR2 stained by GALR2 antibody [C1C2], Internal (GTX100320) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GALR2 antibody [C1C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX100320

GTX100320 IHC-P Image

GALR2 antibody [C1C2], Internal detects GALR2 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
GALR2 stained by GALR2 antibody [C1C2], Internal (GTX100320) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GALR2 antibody [C1C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX100320

GTX100320 IHC-P Image

GALR2 antibody [C1C2], Internal detects GALR2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
GALR2 stained by GALR2 antibody [C1C2], Internal (GTX100320) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GALR2 antibody [C1C2], Internal used in Western Blot (WB). GTX100320

GTX100320 WB Image

Sample(30 ug whole cell lysate)
A:293T
B:H1299
10% SDS PAGE
GTX100320 diluted at 1:1000

Anti-GALR2 antibody [C1C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100320

GTX100320 ICC/IF Image

GALR2 antibody [C1C2], Internal detects GALR2 protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GALR2 stained by GALR2 antibody [C1C2], Internal (GTX100320) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

There are currently no references for GALR2 antibody [C1C2], Internal (GTX100320). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for GALR2 antibody [C1C2], Internal (GTX100320). Be the first to share your experience with this product.
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169