GeneTex
United States (US)

GALR2 antibody

GTX100382_39603_20181115_ICC_IF_R_19011519_226.jpg
GTX100382_WB_19011506_417.jpg
GTX100382_39603_IHC_19011506_976.jpg
GTX100382_39603_IHC_R_19011506_624.jpg

Cat No. GTX100382

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

Positive Control

A431

Predict Reactivity

Mouse, Bovine, Chicken(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 50% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human GALR2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

GALNR2 antibody, MGC125983 antibody, MGC125984 antibody, GALR2 antibody, GALR-2 antibody, GAL2-R antibody, galanin receptor type 2 antibody, galanin receptor 2 antibody

Cellular Localization

Cell membrane; Multi-pass membrane protein

Background

Galanin is an important neuromodulator present in the brain, gastrointestinal system, and hypothalamopituitary axis. It is a 30-amino acid non-C-terminally amidated peptide that potently stimulates growth hormone secretion, inhibits cardiac vagal slowing of heart rate, abolishes sinus arrhythmia, and inhibits postprandial gastrointestinal motility. The actions of galanin are mediated through interaction with specific membrane receptors that are members of the 7-transmembrane family of G protein-coupled receptors. GALR2 interacts with the N-terminal residues of the galanin peptide. The primary signaling mechanism for GALR2 is through the phospholipase C/protein kinase C pathway (via Gq), in contrast to GALR1, which communicates its intracellular signal by inhibition of adenylyl cyclase through Gi. However, it has been demonstrated that GALR2 couples efficiently to both the Gq and Gi proteins to simultaneously activate 2 independent signal transduction pathways. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX100382_39603_20181115_ICC_IF_R_19011519_226.jpg

GTX100382 ICC/IF Image

GALR2 antibody detects GALR2 protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GALR2 stained by GALR2 antibody (GTX100382) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX100382_WB_19011506_417.jpg

GTX100382 WB Image

Sample(30 μg of whole cell lysate)
A:A431(GTX27909)
12% SDS PAGE
GTX100382 diluted at 1:500

GTX100382_39603_IHC_19011506_976.jpg

GTX100382 IHC-P Image

Galanin Receptor 2 antibody detects GALR2 protein at membrane on Cal27 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded Cal27 xenograft.
Galanin Receptor 2 antibody (GTX100382) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100382_39603_IHC_R_19011506_624.jpg

GTX100382 IHC-P Image

Galanin Receptor 2 antibody detects Galanin Receptor 2 protein at membrane and cytosol on rat fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat fore brain.
Galanin Receptor 2 antibody (GTX100382) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169