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GALR2 antibody

Anti-GALR2 antibody used in Western Blot (WB). GTX100382
Anti-GALR2 antibody used in IHC (Paraffin sections) (IHC-P). GTX100382
Anti-GALR2 antibody used in IHC (Paraffin sections) (IHC-P). GTX100382
Anti-GALR2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100382

Cat. No. GTX100382

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Rat
Package
100 μl ($399),
25 μl ($169)
See all GALR2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

Positive Control

A431

Predict Reactivity

Mouse, Bovine, Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide encompassing a sequence within the Intracellular domain of human GALR2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1240879

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

galanin receptor 2 , GAL2-R , GALNR2 , GALR-2

Cellular Localization

Cell membrane; Multi-pass membrane protein

Background

Galanin is an important neuromodulator present in the brain, gastrointestinal system, and hypothalamopituitary axis. It is a 30-amino acid non-C-terminally amidated peptide that potently stimulates growth hormone secretion, inhibits cardiac vagal slowing of heart rate, abolishes sinus arrhythmia, and inhibits postprandial gastrointestinal motility. The actions of galanin are mediated through interaction with specific membrane receptors that are members of the 7-transmembrane family of G protein-coupled receptors. GALR2 interacts with the N-terminal residues of the galanin peptide. The primary signaling mechanism for GALR2 is through the phospholipase C/protein kinase C pathway (via Gq), in contrast to GALR1, which communicates its intracellular signal by inhibition of adenylyl cyclase through Gi. However, it has been demonstrated that GALR2 couples efficiently to both the Gq and Gi proteins to simultaneously activate 2 independent signal transduction pathways. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-GALR2 antibody used in Western Blot (WB). GTX100382

GTX100382 WB Image

Sample(30 μg of whole cell lysate)
A:A431(GTX27909)
12% SDS PAGE
GTX100382 diluted at 1:500

Anti-GALR2 antibody used in IHC (Paraffin sections) (IHC-P). GTX100382

GTX100382 IHC-P Image

Galanin Receptor 2 antibody detects GALR2 protein at membrane on Cal27 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded Cal27 xenograft.
Galanin Receptor 2 antibody (GTX100382) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-GALR2 antibody used in IHC (Paraffin sections) (IHC-P). GTX100382

GTX100382 IHC-P Image

Galanin Receptor 2 antibody detects Galanin Receptor 2 protein at membrane and cytosol on rat fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat fore brain.
Galanin Receptor 2 antibody (GTX100382) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-GALR2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100382

GTX100382 ICC/IF Image

GALR2 antibody detects GALR2 protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GALR2 stained by GALR2 antibody (GTX100382) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

Application Reference

Kiezun J et al. Biomolecules 2022; 12 (12) Galanin Receptors (GALR1, GALR2, and GALR3) Immunoexpression in Enteric Plexuses of Colorectal Cancer Patients: Correlation with the Clinico-Pathological Parameters.
Application : IHC-P
Reactivity : Human
Jacek Kiezun et al. Int J Mol Sci 2022; 23 (7) Galanin Receptors (GalR1, GalR2, and GalR3) Expression in Colorectal Cancer Tissue and Correlations to the Overall Survival and Poor Prognosis of CRC Patients.
Application : IHC-P
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169