GeneTex
United States (US)

GALR3 antibody

GTX108163_39764_20171229_WB_M_eye_19011507_528.jpg
GTX108163_39764_20171229_WB_M_R_19011507_963.jpg
GTX108163_IHC_19011507_149.jpg
GTX108163_WB_19011507_403.jpg

Cat No. GTX108163

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, IHC
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IHC Assay dependent
Not tested in other applications.

Calculated MW

40 kDa. ( Note )

Positive Control

A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji , mouse eye , mouse brain , rat brain
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.72 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human GALR3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Galanin Receptor 3,Galr3

Background

The neuropeptide galanin modulates a variety of physiologic processes including cognition/memory, sensory/pain processing, hormone secretion, and feeding behavior. The human galanin receptors are G protein-coupled receptors that functionally couple to their intracellular effector through distinct signaling pathways. GALR3 is found in many tissues and may be expressed as 1.4-, 2.4-, and 5-kb transcripts [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX108163_39764_20171229_WB_M_eye_19011507_528.jpg

GTX108163 WB Image

Mouse tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with GALR3 antibody (GTX108163) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX108163_39764_20171229_WB_M_R_19011507_963.jpg

GTX108163 WB Image

Various tissue extracts (50 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with GALR3 antibody (GTX108163) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX108163_IHC_19011507_149.jpg

GTX108163 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using GALR3(GTX108163) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX108163_WB_19011507_403.jpg

GTX108163 WB Image

GALR3 antibody detects GALR3 protein by Western blot analysis.
A. 30 µg HeLa whole cell lysate/extract
B. 30 µg Raji whole cell lysate/extract
10 % SDS-PAGE
GALR3 antibody (GTX108163) dilution: 1:1000

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169