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GAP43 antibody

Anti-GAP43 antibody used in Western Blot (WB). GTX04779
Anti-GAP43 antibody used in IHC (Paraffin sections) (IHC-P). GTX04779
Anti-GAP43 antibody used in Flow cytometry (FACS). GTX04779

Cat. No. GTX04779

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, FACS, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μg ($469)
See all GAP43 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.1-0.25 μg/mL
IHC-P 0.5-1 μg/mL
FACS 1-3 μg/10⁶ cells
ELISA 0.1-0.5 μg/mL
Not tested in other applications.

Calculated MW

24 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄

Preservative

0.01 mg sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

E.coli-derived mouse GAP43 recombinant protein (Position: M1-A227).

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

growth associated protein 43 , B-50 , Basp2 , GAP-43

Cellular Localization

Cell junction, synapse

Background

Predicted to enable calmodulin binding activity and phospholipid binding activity. Acts upstream of or within axon choice point recognition; cell fate commitment; and glial cell differentiation. Located in several cellular components, including axon; plasma membrane; and postsynaptic density. Is expressed in several structures, including alimentary system; future brain; genitourinary system; nervous system; and sensory organ. Orthologous to human GAP43 (growth associated protein 43). [provided by Alliance of Genome Resources, Apr 2022]

Database

Research Area

DATA IMAGES

Anti-GAP43 antibody used in Western Blot (WB). GTX04779

GTX04779 WB Image

WB analysis of various samples using GTX04779 GAP43 antibody.
Loading : 50 μg
Dilution : 0.25 μg/mL

Anti-GAP43 antibody used in IHC (Paraffin sections) (IHC-P). GTX04779

GTX04779 IHC-P Image

IHC-P analysis of rat brain tissue using GTX04779 GAP43 antibody.
Antigen retrieval : Heat mediated EDTA buffer (pH 8.0)
Dilution : 1 μg/mL

Anti-GAP43 antibody used in Flow cytometry (FACS). GTX04779

GTX04779 FACS Image

FACS analysis of Raji cells using GTX04779 GAP43 antibody.
Blue : Primary antibody
Green : Isotype control
Red : Unlabelled sample
Dilution : 1 μg/10⁶ cells

REFERENCE

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REVIEW

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Package List Price ($)
$ 469