GeneTex
United States (US)

GAP43 antibody

GTX101535_40016_20170705_IFA_R_19011506_926.jpg
GTX101535_40016_20170511_WB_M_hippocampus_19011506_239.jpg
GTX101535_40016_20170511_WB_R_hippocampus_19011506_588.jpg
GTX101535_40016_WB_19011506_277.jpg

Cat No. GTX101535

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

25 kDa. ( Note )

Positive Control

IMR32 , mouse hippocampus , rat hippocampus

Predict Reactivity

Bovine, Cat, Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.55 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human GAP43. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Growth Associated Protein 43,B-50,Pp46,Gap43

Cellular Localization

Cell membrane; Peripheral membrane protein; Cytoplasmic side , Cell projection , growth cone membrane; Peripheral membrane protein; Cytoplasmic side , Cell junction , synapse

Background

The protein encoded by this gene has been termed a 'growth' or 'plasticity' protein because it is expressed at high levels in neuronal growth cones during development and axonal regeneration. This protein is considered a crucial component of an effective regenerative response in the nervous system. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX101535_40016_20170705_IFA_R_19011506_926.jpg

GTX101535 ICC/IF Image

GAP43 antibody detects GAP43 protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GAP43 protein stained by GAP43 antibody (GTX101535) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT886] (GTX631830) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX101535_40016_20170511_WB_M_hippocampus_19011506_239.jpg

GTX101535 WB Image

Mouse tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with GAP43 antibody (GTX101535) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101535_40016_20170511_WB_R_hippocampus_19011506_588.jpg

GTX101535 WB Image

Rat tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with GAP43 antibody (GTX101535) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101535_40016_WB_19011506_277.jpg

GTX101535 WB Image

Sample (30 ug of whole cell lysate)
A: IMR32
12% SDS PAGE
GTX101535 diluted at 1:1000

REVIEW

GAP43 antibody Cat No. GTX101535

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 14-Feb-2012
Score :
Application Tested : WB
Sample Species : Rat
Sample : Rat brain
Amount used : 37.5μg
Blocking : 5% non-fat milk, 4°C, 16Hr
Primary Antibody : 1:1000, 4°C, 8Hr
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169