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GAP43 antibody

Anti-GAP43 antibody used in Western Blot (WB). GTX127937
Anti-GAP43 antibody used in Western Blot (WB). GTX127937
Anti-GAP43 antibody used in IHC (Frozen sections) (IHC-Fr). GTX127937
Anti-GAP43 antibody used in Western Blot (WB). GTX127937
Anti-GAP43 antibody used in Western Blot (WB). GTX127937
Anti-GAP43 antibody used in Western Blot (WB). GTX127937
Anti-GAP43 antibody used in IHC (Paraffin sections) (IHC-P). GTX127937
Anti-GAP43 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX127937

Cat No. GTX127937

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:30000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

24 kDa. ( Note )

Positive Control

SK-N-SH , IMR32 , SK-N-AS , mouse fetal brain , mouse hippocampus , rat hippocampus , mouse brain , rat brain

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of rat GAP43. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

growth associated protein 43 , Basp2

Cellular Localization

Cell membrane; Peripheral membrane protein; Cytoplasmic side , Cell projection , growth cone membrane; Peripheral membrane protein; Cytoplasmic side , Cell junction , synapse

Background

may play a role in neurite outgrowth and nerve regeneration [RGD]

Database

Research Area

DATA IMAGES

Anti-GAP43 antibody used in Western Blot (WB). GTX127937

GTX127937 WB Image

Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with GAP43 antibody (GTX127937) diluted at 1:30000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GAP43 antibody used in Western Blot (WB). GTX127937

GTX127937 WB Image

GAP43 antibody detects Gap43 protein by western blot analysis.
A. 30 μg SK-N-SH whole cell lysate/extract
B. 30 μg IMR32 whole cell lysate/extract
C. 30 μg SK-N-AS whole cell lysate/extract
12% SDS-PAGE
GAP43 antibody (GTX127937) dilution: 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GAP43 antibody used in IHC (Frozen sections) (IHC-Fr). GTX127937

GTX127937 IHC-Fr Image

GAP43 antibody detects GAP43 protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse hippocampus.
Green: GAP43 protein stained by GAP43 antibody (GTX127937) diluted at 1:250.
Red: NeuN, stained by NeuN antibody [2Q158] (GTX30773) diluted at 1:500.

Anti-GAP43 antibody used in Western Blot (WB). GTX127937

GTX127937 WB Image

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with GAP43 antibody (GTX127937) diluted at 1:30000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GAP43 antibody used in Western Blot (WB). GTX127937

GTX127937 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GAP43 antibody (GTX127937) diluted at 1:30000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GAP43 antibody used in Western Blot (WB). GTX127937

GTX127937 WB Image

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with GAP43 antibody (GTX127937) diluted at 1:30000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GAP43 antibody used in IHC (Paraffin sections) (IHC-P). GTX127937

GTX127937 IHC-P Image

GAP43 antibody detects Gap43 protein at on Cal27 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded Cal27 xenograft.
GAP43 antibody (GTX127937) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-GAP43 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX127937

GTX127937 ICC/IF Image

GAP43 antibody detects GAP43 protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GAP43 protein stained by GAP43 antibody (GTX127937) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT886] (GTX631830) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169