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GAP43 antibody

Orthogonal
Anti-GAP43 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30199
Anti-GAP43 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30199
Anti-GAP43 antibody used in Western Blot (WB). GTX30199
Anti-GAP43 antibody used in IHC (Frozen sections) (IHC-Fr). GTX30199

Cat. No. GTX30199

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr, ELISA

Reactivity

 Human, Mouse, Rat, Bovine, Dog, Chicken, Pig, Primate
Package
100 μl ($609)
See all GAP43 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:10000
ICC/IF 1:1000
IHC-P 1 - 2 μg/ml
IHC-Fr 1 - 2 μg/ml
ELISA Assay dependent
Not tested in other applications.

Calculated MW

24 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

5mM Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Immunogen

C-terminal peptide of rat and mouse GAP43, which is KEDPEADQEHA, with an N-terminal Cys added to allow chemical coupling to KLH carrier protein.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_371434

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

growth associated protein 43 , Basp2

Cellular Localization

Cell junction, synapse

Background

may play a role in neurite outgrowth and nerve regeneration [RGD]

Database

Research Area

DATA IMAGES

Anti-GAP43 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30199

GTX30199 ICC/IF Image

ICC/IF analysis of rat E18 mixed neuron/glia cell cultures using GTX30199 GAP43 antibody.
Red : GAP43 antibody
Green : MAP-tau antibody
Blue : DAPI

Anti-GAP43 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30199

GTX30199 ICC/IF Image

ICC/IF analysis of mixed neuron/glia cell cultures using GTX30199 GAP43 antibody.
Red : primary antibody
Blue : DAPI

Anti-GAP43 antibody used in Western Blot (WB). GTX30199

GTX30199 WB Image

WB analysis of cow cerebellum tissue using GTX30199 GAP43 antibody.

Anti-GAP43 antibody used in IHC (Frozen sections) (IHC-Fr). GTX30199

GTX30199 IHC-Fr Image

Orthogonal

The data was published in the journal Front Neurosci in 2018.PMID: 30618589

REFERENCE

Application Reference

Xu Z et al. Front Neurosci 2018; 12 : 970 Retinal Neuroprotection From Optic Nerve Trauma by Deletion of Arginase 2.
Gnanapavan S et al. J Neuroimmunol 2014; (Epub) Growth Associated Protein (GAP-43): cloning and the development of a sensitive ELISA for neurological disorders
Application : ELISA
Chang YJ et al. Mol Cell Biol 2014; 34 (6) : 1003-19 SH2B1β Interacts with STAT3 and Enhances Fibroblast Growth Factor 1-Induced Gene Expression during Neuronal Differentiation.
Application : WB
Reactivity : Rat
Submit a Reference

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 609