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GAP43 antibody

Anti-GAP43 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50592
Anti-GAP43 antibody used in Western Blot (WB). GTX50592
Anti-GAP43 antibody used in IHC (Paraffin sections) (IHC-P). GTX50592

Cat No. GTX50592

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:1000
ICC/IF 1:100-1:200
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

25 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH 7.4, 150mM NaCl, 0.02% sodium azide, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around aa.38~43 (Q-A-S-F-R) derived from human GAP43.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

growth associated protein 43 , B-50 , PP46

Cellular Localization

Cell membrane,Cell junction, synapse

Background

The protein encoded by this gene has been termed a 'growth' or 'plasticity' protein because it is expressed at high levels in neuronal growth cones during development and axonal regeneration. This protein is considered a crucial component of an effective regenerative response in the nervous system. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-GAP43 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50592

GTX50592 ICC/IF Image

ICC/IF analysis of methanol-fixed HeLa cells using GTX50592 GAP43 antibody.

Anti-GAP43 antibody used in Western Blot (WB). GTX50592

GTX50592 WB Image

WB analysis of extracts from mouse brain tissue using GTX50592 GAP43 antibody with or without blocking peptide.

Anti-GAP43 antibody used in IHC (Paraffin sections) (IHC-P). GTX50592

GTX50592 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX50592 GAP43 antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 319