APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 0.01-0.5μg/ml |
| 5μg/ml |
| 5μg/ml |
|
Note :
ICC/IF
Strong expression of the protein seen in the cytoplasm of HeLa cells.
|
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX89740-PEP).
Predict Reactivity
Dog, Pig(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-HQVVSSDFNSDT, from the C Terminus of the protein sequence according to NP_002037.2.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
RRID
AB_10721581
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
glyceraldehyde-3-phosphate dehydrogenase , G3PD , GAPD , HEL-S-162eP
Cellular Localization
Cytoplasm, cytosol , Nucleus , Membrane , Cytoplasm, cytoskeleton
Background
This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Many pseudogenes similar to this locus are present in the human genome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2014]
Database
Research Area
DATA IMAGES
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GTX89740 ICC/IF Image
ICC/IF analysis of PFA fixed HeLa cells using GTX89740 GAPDH antibody, C-term.
Green : Primary antibody
Blue : DAPI
Negative control : Unimmunized goat IgG
Permeabilization : 0.15% Triton
Dilution : 5μg/ml
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GTX89740 IHC-P Image
IHC-P analysis of human kidney using GTX89740 GAPDH antibody, C-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 5μg/ml
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GTX89740 WB Image
WB analysis of mouse liver (A) and rat brain (B) lysate using GTX89740 GAPDH antibody, C-term.
Dilution : 0.5μg/ml
Loading : 35μg protein in RIPA buffer
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GTX89740 WB Image
WB analysis of human liver (A) and tonsil (B) lysate using GTX89740 GAPDH antibody, C-term.
Dilution : 0.1μg/ml
Loading : 35μg protein in RIPA buffer
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GTX89740 WB Image
WB analysis of HEK293 (A) and HeLa (B) cell lysate using GTX89740 GAPDH antibody, C-term.
Dilution : 0.1μg/ml
Loading : 35μg protein in RIPA buffer
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REFERENCE
REVIEW
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