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GAPDH antibody [GT1355]

Anti-GAPDH antibody [GT1355] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX03407
Anti-GAPDH antibody [GT1355] used in Western Blot (WB). GTX03407
Anti-GAPDH antibody [GT1355] used in Western Blot (WB). GTX03407
Anti-GAPDH antibody [GT1355] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX03407
Anti-GAPDH antibody [GT1355] used in Western Blot (WB). GTX03407
Anti-GAPDH antibody [GT1355] used in IHC (Paraffin sections) (IHC-P). GTX03407

Cat. No. GTX03407

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 GT1355

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($419)
See all GAPDH products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human GAPDH

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glyceraldehyde-3-phosphate dehydrogenase , G3PD , GAPD , HEL-S-162eP

Cellular Localization

Cytoplasm, cytosol , Nucleus , Membrane , Cytoplasm, cytoskeleton

Background

This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Many pseudogenes similar to this locus are present in the human genome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2014]

Database

Research Area

DATA IMAGES

Anti-GAPDH antibody [GT1355] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX03407

GTX03407 ICC/IF Image

ICC/IF analysis of NIH-3T3 cells using GTX03407 GAPDH antibody [GT1355].
Red : Primary antibody
Blue : DAPI for nuclear staining
Dilution : 1:100

Anti-GAPDH antibody [GT1355] used in Western Blot (WB). GTX03407

GTX03407 WB Image

WB analysis of HeLa cell lysates using serially diluted GTX03407 GAPDH antibody [GT1355].
Loading : 25μg per lane

Anti-GAPDH antibody [GT1355] used in Western Blot (WB). GTX03407

GTX03407 WB Image

WB analysis of various sample lysates using GTX03407 GAPDH antibody [GT1355].
Dilution : 1:1000
Loading : 25μg per lane

Anti-GAPDH antibody [GT1355] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX03407

GTX03407 ICC/IF Image

ICC/IF analysis of C6 cells using GTX03407 GAPDH antibody [GT1355].
Red : Primary antibody
Blue : DAPI for nuclear staining
Dilution : 1:100

Anti-GAPDH antibody [GT1355] used in Western Blot (WB). GTX03407

GTX03407 WB Image

WB analysis of various sample lysates using GTX03407 GAPDH antibody [GT1355].
Dilution : 1:10000
Loading : 25μg per lane

Anti-GAPDH antibody [GT1355] used in IHC (Paraffin sections) (IHC-P). GTX03407

GTX03407 IHC-P Image

IHC-P analysis of human placenta tissue using GTX03407 GAPDH antibody [GT1355].
Antigen retrieval : Microwave antigen retrieval with 10 mM PBS buffer pH 7.2
Dilution : 1:100

REFERENCE

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REVIEW

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Package List Price ($)
$ 419