APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Positive Control
MDCK
Predict Reactivity
Human, Mouse, Rat, Zebrafish, Rabbit, Sheep, Bovine, Chicken, Guinea pig, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No Preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Immunogen
Recombinant protein corresponding to dog GAPDH.
Purification
Affinity purified by Protein G.
Conjugation
Unconjugated
RRID
AB_2888497
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
glyceraldehyde-3-phosphate dehydrogenase
Database
Research Area
DATA IMAGES
![Anti-GAPDH antibody [GT239] - VetSignal used in Western Blot (WB). GTX635013](/templates/default/js/lightbox/images/loading.gif "Anti-GAPDH antibody [GT239] - VetSignal used in Western Blot (WB). GTX635013")
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GTX635013 WB Image
Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with GAPDH antibody (GTX635013) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX635013 IHC-P Image
GAPDH antibody [GT239] detects GAPDH protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded dog tongue.GAPDH stained by GAPDH antibody [GT239] (GTX635013) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX635013 ICC/IF Image
GAPDH antibody [GT239] detects GAPDH protein at cytoplasm by immunofluorescent analysis.Sample: MDCK cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: GAPDH stained by GAPDH antibody [GT239] (GTX635013) diluted at 1:500.Blue: Hoechst 33342 staining.
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GTX635013 WB Image
Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with GAPDH antibody (GTX635013) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX635013 IHC-P Image
GAPDH antibody detects GAPDH protein at cytoplasm and nucleus by immunohistochemical analysis.Sample: Paraffin-embedded cat liver.GAPDH stained by GAPDH antibody (GTX635013) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX635013 IHC-P Image
GAPDH antibody detects GAPDH protein at cytoplasm and nucleus by immunohistochemical analysis.Sample: Paraffin-embedded cat kidney.GAPDH stained by GAPDH antibody (GTX635013) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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REFERENCE
There are currently no references for GAPDH antibody [GT239] - VetSignal (GTX635013). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for GAPDH antibody [GT239] - VetSignal (GTX635013). Be the first to share your experience with this product.
![Anti-GAPDH antibody [GT239] - VetSignal used in Western Blot (WB). GTX635013](/upload/website/prouct_img/small/GTX635013/GTX635013_41323_20181123_WB_D_19011519_168.jpg "Anti-GAPDH antibody [GT239] - VetSignal used in Western Blot (WB). GTX635013")
![Anti-GAPDH antibody [GT239] - VetSignal used in IHC (Paraffin sections) (IHC-P). GTX635013](/upload/website/prouct_img/small/GTX635013/GTX635013_41323_20190215_IHC-P_D_19030801_983.jpg "Anti-GAPDH antibody [GT239] - VetSignal used in IHC (Paraffin sections) (IHC-P). GTX635013")
![Anti-GAPDH antibody [GT239] - VetSignal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635013](/upload/website/prouct_img/small/GTX635013/GTX635013_41323_20190612_ICC_IF_D_19072822_877.jpg "Anti-GAPDH antibody [GT239] - VetSignal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635013")
![Anti-GAPDH antibody [GT239] - VetSignal used in Western Blot (WB). GTX635013](/upload/website/prouct_img/small/GTX635013/GTX635013_41323_20190412_WB_C_19052000_340.jpg "Anti-GAPDH antibody [GT239] - VetSignal used in Western Blot (WB). GTX635013")
![Anti-GAPDH antibody [GT239] - VetSignal used in IHC (Paraffin sections) (IHC-P). GTX635013](/upload/website/prouct_img/small/GTX635013/GTX635013_41323_20190719_IHC-P_C_19072822_750.jpg "Anti-GAPDH antibody [GT239] - VetSignal used in IHC (Paraffin sections) (IHC-P). GTX635013")
![Anti-GAPDH antibody [GT239] - VetSignal used in IHC (Paraffin sections) (IHC-P). GTX635013](/upload/website/prouct_img/small/GTX635013/GTX635013_41323_20190719_IHC-P_C_1_19072822_941.jpg "Anti-GAPDH antibody [GT239] - VetSignal used in IHC (Paraffin sections) (IHC-P). GTX635013")
![Anti-GAPDH antibody [GT239] - VetSignal used in Western Blot (WB). GTX635013](/upload/website/prouct_img/normal/GTX635013/GTX635013_41323_20181123_WB_D_19011519_168.jpg "Anti-GAPDH antibody [GT239] - VetSignal used in Western Blot (WB). GTX635013")
![Anti-GAPDH antibody [GT239] - VetSignal used in IHC (Paraffin sections) (IHC-P). GTX635013](/upload/website/prouct_img/normal/GTX635013/GTX635013_41323_20190215_IHC-P_D_19030801_983.jpg "Anti-GAPDH antibody [GT239] - VetSignal used in IHC (Paraffin sections) (IHC-P). GTX635013")
![Anti-GAPDH antibody [GT239] - VetSignal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635013](/upload/website/prouct_img/normal/GTX635013/GTX635013_41323_20190612_ICC_IF_D_19072822_877.jpg "Anti-GAPDH antibody [GT239] - VetSignal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635013")
![Anti-GAPDH antibody [GT239] - VetSignal used in Western Blot (WB). GTX635013](/upload/website/prouct_img/normal/GTX635013/GTX635013_41323_20190412_WB_C_19052000_340.jpg "Anti-GAPDH antibody [GT239] - VetSignal used in Western Blot (WB). GTX635013")
![Anti-GAPDH antibody [GT239] - VetSignal used in IHC (Paraffin sections) (IHC-P). GTX635013](/upload/website/prouct_img/normal/GTX635013/GTX635013_41323_20190719_IHC-P_C_19072822_750.jpg "Anti-GAPDH antibody [GT239] - VetSignal used in IHC (Paraffin sections) (IHC-P). GTX635013")
![Anti-GAPDH antibody [GT239] - VetSignal used in IHC (Paraffin sections) (IHC-P). GTX635013](/upload/website/prouct_img/normal/GTX635013/GTX635013_41323_20190719_IHC-P_C_1_19072822_941.jpg "Anti-GAPDH antibody [GT239] - VetSignal used in IHC (Paraffin sections) (IHC-P). GTX635013")