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GAPDH antibody [GT239] - VetSignal

Anti-GAPDH antibody [GT239] - VetSignal used in IHC (Paraffin sections) (IHC-P). GTX635013
Anti-GAPDH antibody [GT239] - VetSignal used in IHC (Paraffin sections) (IHC-P). GTX635013
Anti-GAPDH antibody [GT239] - VetSignal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635013
Anti-GAPDH antibody [GT239] - VetSignal used in IHC (Paraffin sections) (IHC-P). GTX635013
Anti-GAPDH antibody [GT239] - VetSignal used in Western Blot (WB). GTX635013
Anti-GAPDH antibody [GT239] - VetSignal used in Western Blot (WB). GTX635013

Cat No. GTX635013

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT239

Isotype

 IgG2b

Application

 WB, ICC/IF, IHC-P

Reactivity

 Cat, Dog
Package
100 μl ($379)
See all GAPDH products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Predict Reactivity

Human, Mouse, Rat, Zebrafish, Rabbit, Sheep, Bovine, Chicken, Guinea pig, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Recombinant protein corresponding to dog GAPDH.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888497

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

glyceraldehyde-3-phosphate dehydrogenase

Database

Research Area

DATA IMAGES

Anti-GAPDH antibody [GT239] - VetSignal used in IHC (Paraffin sections) (IHC-P). GTX635013

GTX635013 IHC-P Image

GAPDH antibody detects GAPDH protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded cat liver.
GAPDH stained by GAPDH antibody (GTX635013) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GAPDH antibody [GT239] - VetSignal used in IHC (Paraffin sections) (IHC-P). GTX635013

GTX635013 IHC-P Image

GAPDH antibody detects GAPDH protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded cat kidney.
GAPDH stained by GAPDH antibody (GTX635013) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GAPDH antibody [GT239] - VetSignal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635013

GTX635013 ICC/IF Image

GAPDH antibody [GT239] detects GAPDH protein at cytoplasm by immunofluorescent analysis.
Sample: MDCK cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GAPDH stained by GAPDH antibody [GT239] (GTX635013) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-GAPDH antibody [GT239] - VetSignal used in IHC (Paraffin sections) (IHC-P). GTX635013

GTX635013 IHC-P Image

GAPDH antibody [GT239] detects GAPDH protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded dog tongue.
GAPDH stained by GAPDH antibody [GT239] (GTX635013) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GAPDH antibody [GT239] - VetSignal used in Western Blot (WB). GTX635013

GTX635013 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with GAPDH antibody (GTX635013) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-GAPDH antibody [GT239] - VetSignal used in Western Blot (WB). GTX635013

GTX635013 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with GAPDH antibody (GTX635013) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Natascha Leitner et al. BMC Vet Res 2022; 18 (1) : 221 Lipid droplet dynamics in healthy and pyometra-affected canine endometrium.
Application : WB
Reactivity : Dog
Submit a Reference

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SDS
PBS.pdf
Package List Price ($)
$ 379
Custom Conjugations