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GAPDH antibody [GT239] - VetSignal

Anti-GAPDH antibody [GT239] - VetSignal used in IHC (Paraffin sections) (IHC-P). GTX635013
Anti-GAPDH antibody [GT239] - VetSignal used in IHC (Paraffin sections) (IHC-P). GTX635013
Anti-GAPDH antibody [GT239] - VetSignal used in Western Blot (WB). GTX635013
Anti-GAPDH antibody [GT239] - VetSignal used in IHC (Paraffin sections) (IHC-P). GTX635013
Anti-GAPDH antibody [GT239] - VetSignal used in Western Blot (WB). GTX635013
Anti-GAPDH antibody [GT239] - VetSignal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635013

Cat No. GTX635013

Host Mouse
Clonality Monoclonal
Clone Name GT239
Isotype IgG2b
Application WB, ICC/IF, IHC-P
Reactivity Cat, Dog
Package
100 μl ($319)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Positive Control

MDCK

Predict Reactivity

Human, Mouse, Rat, Zebrafish, Rabbit, Sheep, Bovine, Chicken, Guinea pig, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20º C or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Recombinant protein corresponding to dog GAPDH.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

glyceraldehyde-3-phosphate dehydrogenase

Database

Research Area

DATA IMAGES
Anti-GAPDH antibody [GT239] - VetSignal used in IHC (Paraffin sections) (IHC-P). GTX635013

GTX635013 IHC-P Image

GAPDH antibody detects GAPDH protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded cat liver.
GAPDH stained by GAPDH antibody (GTX635013) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GAPDH antibody [GT239] - VetSignal used in IHC (Paraffin sections) (IHC-P). GTX635013

GTX635013 IHC-P Image

GAPDH antibody detects GAPDH protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded cat kidney.
GAPDH stained by GAPDH antibody (GTX635013) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GAPDH antibody [GT239] - VetSignal used in Western Blot (WB). GTX635013

GTX635013 WB Image

Whole cell extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with GAPDH antibody (GTX635013) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-GAPDH antibody [GT239] - VetSignal used in IHC (Paraffin sections) (IHC-P). GTX635013

GTX635013 IHC-P Image

GAPDH antibody [GT239] detects GAPDH protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded dog tongue.
GAPDH stained by GAPDH antibody [GT239] (GTX635013) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GAPDH antibody [GT239] - VetSignal used in Western Blot (WB). GTX635013

GTX635013 WB Image

Whole cell extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with GAPDH antibody (GTX635013) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-GAPDH antibody [GT239] - VetSignal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635013

GTX635013 ICC/IF Image

GAPDH antibody [GT239] detects GAPDH protein at cytoplasm by immunofluorescent analysis.
Sample: MDCK cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GAPDH stained by GAPDH antibody [GT239] (GTX635013) diluted at 1:500.
Blue: Hoechst 33342 staining.

REFERENCE
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SDS
PBS.pdf
Package List Price ($)
$ 319