APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Positive Control
MDCK
Predict Reactivity
Human, Mouse, Rat, Zebrafish, Rabbit, Sheep, Bovine, Chicken, Guinea pig, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20º C or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Immunogen
Recombinant protein corresponding to dog GAPDH.
Purification
Affinity purified by Protein G.
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
glyceraldehyde-3-phosphate dehydrogenase
Database
Research Area
DATA IMAGES
![Anti-GAPDH antibody [GT239] - VetSignal used in IHC (Paraffin sections) (IHC-P). GTX635013](/upload/website/prouct_img/middle/GTX635013/GTX635013_41323_20190215_IHC-P_D_19030801_983.jpg "Anti-GAPDH antibody [GT239] - VetSignal used in IHC (Paraffin sections) (IHC-P). GTX635013")
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GTX635013 IHC-P Image
GAPDH antibody [GT239] detects GAPDH protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded dog tongue.GAPDH stained by GAPDH antibody [GT239] (GTX635013) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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![Anti-GAPDH antibody [GT239] - VetSignal used in Western Blot (WB). GTX635013](/upload/website/prouct_img/middle/GTX635013/GTX635013_41323_20190412_WB_C_19052000_340.jpg "Anti-GAPDH antibody [GT239] - VetSignal used in Western Blot (WB). GTX635013")
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GTX635013 WB Image
Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with GAPDH antibody (GTX635013) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
|
![Anti-GAPDH antibody [GT239] - VetSignal used in Western Blot (WB). GTX635013](/upload/website/prouct_img/middle/GTX635013/GTX635013_41323_20181123_WB_D_19011519_168.jpg "Anti-GAPDH antibody [GT239] - VetSignal used in Western Blot (WB). GTX635013")
|
GTX635013 WB Image
Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with GAPDH antibody (GTX635013) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
|
![Anti-GAPDH antibody [GT239] - VetSignal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635013](/upload/website/prouct_img/middle/GTX635013/GTX635013_41323_20190612_ICC_IF_D_19072822_877.jpg "Anti-GAPDH antibody [GT239] - VetSignal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635013")
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GTX635013 ICC/IF Image
GAPDH antibody [GT239] detects GAPDH protein at cytoplasm by immunofluorescent analysis.Sample: MDCK cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: GAPDH stained by GAPDH antibody [GT239] (GTX635013) diluted at 1:500.Blue: Hoechst 33342 staining.
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![Anti-GAPDH antibody [GT239] - VetSignal used in IHC (Paraffin sections) (IHC-P). GTX635013](/upload/website/prouct_img/middle/GTX635013/GTX635013_41323_20190719_IHC-P_C_19072822_750.jpg "Anti-GAPDH antibody [GT239] - VetSignal used in IHC (Paraffin sections) (IHC-P). GTX635013")
|
GTX635013 IHC-P Image
GAPDH antibody detects GAPDH protein at cytoplasm and nucleus by immunohistochemical analysis.Sample: Paraffin-embedded cat liver.GAPDH stained by GAPDH antibody (GTX635013) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
|
![Anti-GAPDH antibody [GT239] - VetSignal used in IHC (Paraffin sections) (IHC-P). GTX635013](/upload/website/prouct_img/middle/GTX635013/GTX635013_41323_20190719_IHC-P_C_1_19072822_941.jpg "Anti-GAPDH antibody [GT239] - VetSignal used in IHC (Paraffin sections) (IHC-P). GTX635013")
|
GTX635013 IHC-P Image
GAPDH antibody detects GAPDH protein at cytoplasm and nucleus by immunohistochemical analysis.Sample: Paraffin-embedded cat kidney.GAPDH stained by GAPDH antibody (GTX635013) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
|
REFERENCE
There are currently no references for GAPDH antibody [GT239] - VetSignal (GTX635013). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for GAPDH antibody [GT239] - VetSignal (GTX635013). Be the first to share your experience with this product.
![Anti-GAPDH antibody [GT239] - VetSignal used in IHC (Paraffin sections) (IHC-P). GTX635013](/upload/website/prouct_img/small/GTX635013/GTX635013_41323_20190215_IHC-P_D_19030801_983.jpg "Anti-GAPDH antibody [GT239] - VetSignal used in IHC (Paraffin sections) (IHC-P). GTX635013")
![Anti-GAPDH antibody [GT239] - VetSignal used in Western Blot (WB). GTX635013](/upload/website/prouct_img/small/GTX635013/GTX635013_41323_20190412_WB_C_19052000_340.jpg "Anti-GAPDH antibody [GT239] - VetSignal used in Western Blot (WB). GTX635013")
![Anti-GAPDH antibody [GT239] - VetSignal used in Western Blot (WB). GTX635013](/upload/website/prouct_img/small/GTX635013/GTX635013_41323_20181123_WB_D_19011519_168.jpg "Anti-GAPDH antibody [GT239] - VetSignal used in Western Blot (WB). GTX635013")
![Anti-GAPDH antibody [GT239] - VetSignal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635013](/upload/website/prouct_img/small/GTX635013/GTX635013_41323_20190612_ICC_IF_D_19072822_877.jpg "Anti-GAPDH antibody [GT239] - VetSignal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635013")
![Anti-GAPDH antibody [GT239] - VetSignal used in IHC (Paraffin sections) (IHC-P). GTX635013](/upload/website/prouct_img/small/GTX635013/GTX635013_41323_20190719_IHC-P_C_19072822_750.jpg "Anti-GAPDH antibody [GT239] - VetSignal used in IHC (Paraffin sections) (IHC-P). GTX635013")
![Anti-GAPDH antibody [GT239] - VetSignal used in IHC (Paraffin sections) (IHC-P). GTX635013](/upload/website/prouct_img/small/GTX635013/GTX635013_41323_20190719_IHC-P_C_1_19072822_941.jpg "Anti-GAPDH antibody [GT239] - VetSignal used in IHC (Paraffin sections) (IHC-P). GTX635013")
![Anti-GAPDH antibody [GT239] - VetSignal used in IHC (Paraffin sections) (IHC-P). GTX635013](/upload/website/prouct_img/normal/GTX635013/GTX635013_41323_20190215_IHC-P_D_19030801_983.jpg "Anti-GAPDH antibody [GT239] - VetSignal used in IHC (Paraffin sections) (IHC-P). GTX635013")
![Anti-GAPDH antibody [GT239] - VetSignal used in Western Blot (WB). GTX635013](/upload/website/prouct_img/normal/GTX635013/GTX635013_41323_20190412_WB_C_19052000_340.jpg "Anti-GAPDH antibody [GT239] - VetSignal used in Western Blot (WB). GTX635013")
![Anti-GAPDH antibody [GT239] - VetSignal used in Western Blot (WB). GTX635013](/upload/website/prouct_img/normal/GTX635013/GTX635013_41323_20181123_WB_D_19011519_168.jpg "Anti-GAPDH antibody [GT239] - VetSignal used in Western Blot (WB). GTX635013")
![Anti-GAPDH antibody [GT239] - VetSignal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635013](/upload/website/prouct_img/normal/GTX635013/GTX635013_41323_20190612_ICC_IF_D_19072822_877.jpg "Anti-GAPDH antibody [GT239] - VetSignal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635013")
![Anti-GAPDH antibody [GT239] - VetSignal used in IHC (Paraffin sections) (IHC-P). GTX635013](/upload/website/prouct_img/normal/GTX635013/GTX635013_41323_20190719_IHC-P_C_19072822_750.jpg "Anti-GAPDH antibody [GT239] - VetSignal used in IHC (Paraffin sections) (IHC-P). GTX635013")
![Anti-GAPDH antibody [GT239] - VetSignal used in IHC (Paraffin sections) (IHC-P). GTX635013](/upload/website/prouct_img/normal/GTX635013/GTX635013_41323_20190719_IHC-P_C_1_19072822_941.jpg "Anti-GAPDH antibody [GT239] - VetSignal used in IHC (Paraffin sections) (IHC-P). GTX635013")
